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AMR surveillance including whole genome sequencing (WGS)\u2010based epidemiology provides ideal resolution to identify and describe AMR gonococcal clones, AMR determinants and populations, which can inform management guidelines and antimicrobial stewardship policies. Our aims were to, for the first time, elucidate the WGS\u2010based epidemiology and characterize AMR determinants of gonococcal strains spreading in Ukraine, 2013\u20132018. Gonococcal isolates (n\u00a0=\u00a0150) from Ternopil and Dnipro, Ukraine (2013\u20132018), were subjected to AMR testing (Etest) for eight antimicrobials and WGS. Overall, 11.3% of isolates were resistant to ciprofloxacin, 6.0% to tetracycline, and 0.7% to benzylpenicillin. No isolates were resistant to azithromycin, spectinomycin, ceftriaxone, or cefixime, but one isolate was bordering resistance to both cephalosporins. Twenty\u2010five MLST STs, 50 NG\u2010MAST STs, and 34 NG\u2010STAR types were identified. The phylogenomic analysis revealed six main clusters, mostly associated with the internationally described multidrug\u2010susceptible gonococcal lineage. Resistance to ciprofloxacin was associated with GyrA S91F and ParC S87R mutations; tetracyclines with <jats:italic>rpsJ<\/jats:italic> V57M and <jats:italic>tetM<\/jats:italic>; penicillins with mosaic <jats:italic>penA<\/jats:italic>\u201034.001 and \u03b2\u2010lactamase; <jats:italic>mtrR<\/jats:italic>; PorB1b G101D, and PBP1 L421P mutations. One isolate of the multidrug\u2010resistant NG\u2010MAST ST1407, MLST ST1901 was found, which was bordering resistance to ceftriaxone and cefixime. The antimicrobial susceptibility of gonococcal strains spreading in Ternopil and Dnipro, Ukraine, in 2013\u20132018 was surprisingly high. Continued and expanded gonococcal AMR surveillance, ideally including WGS, in Ukraine is essential. This could inform action plans and public health policies to control the spread of AMR gonococcal strains in Ukraine.<\/jats:p>","DOI":"10.1111\/apm.13060","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T00:36:00Z","timestamp":1590107760000},"page":"465-475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Genomic epidemiology and antimicrobial resistance determinants of <i>Neisseria gonorrhoeae<\/i> isolates from Ukraine, 2013\u20132018"],"prefix":"10.1111","volume":"128","author":[{"given":"Iryna","family":"Boiko","sequence":"first","affiliation":[{"name":"Department of Functional and Laboratory Diagnostics I. 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