{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:16:56Z","timestamp":1781155016518,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>In this descriptive pilot study, we aim to establish a porcine <jats:italic>Staphylococcus aureus<\/jats:italic> skin infection model by subcutaneous injection (s.c.) of the porcine S54F9 <jats:italic>S. aureus<\/jats:italic> strain in the groin area. Six pigs were used in the study: Five pigs were injected with <jats:italic>S. aureus<\/jats:italic>, inocula ranging from 7\u2009\u00d7\u200910<jats:sup>3<\/jats:sup> to 5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> colony\u2010forming units per kg bodyweight; one pig was injected with saline exclusively. Lesions were recorded up to 6\u2009days postinoculation using clinical evaluation, ultrasound evaluation, microbiology, flow cytometry, and pathology. Inoculation gave rise to lesions ranging from localized skin infection, that is, minute histological changes, intracellular infection, and macroscopic abscess formation with sequestration of soft tissue, to generalized infection and development of disseminated intravascular coagulation necessitating euthanasia only 10\u2009h after inoculation. Ultrasound assessment of maximum width and characteristics was not able to disclose the progress of the local infection. Flow cytometry and immunohistochemistry revealed the participation of \u03b3\u03b4T cells in the immune response. In conclusion, we did see a graded inflammatory response associated with the dose of s.c. inoculated bacteria, which may be useful for studying, in particular, the interaction of bacteria and inflammatory mononuclear cell populations. 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