{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:26:37Z","timestamp":1791678397986,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T00:00:00Z","timestamp":1622937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microbial co\u2010infections may contribute to the pulmonary deterioration in COVID\u201019 patients needing intensive care treatment. The present study portrays the extent of co\u2010infections in COVID\u201019 ICU patients. Conventional culture, molecular detections for atypical aetiologies, QiaStat\u2010Dx\u00ae respiratory panel V2 detecting 21 respiratory pathogens and ribosomal DNA genes 16S\/18S amplicon\u2010based microbiome analyses were performed on respiratory samples from 34 COVID\u201019 patients admitted to the ICU. Potential pathogens were detected in seven patients (21%) by culturing, in four patients (12%) by microbiome analysis and in one patient (3%) by respiratory panel. Among 20 patients receiving antibiotics prior to ICU admission, fungi (3 <jats:italic>Candida<\/jats:italic> <jats:italic>albicans<\/jats:italic>, 1 <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>tropicalis<\/jats:italic>, 1 <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>dubliniensis<\/jats:italic>) were cultured in 5 (15%) endotracheal aspirates. Among 14 patients who were antibiotic\u2010naive at ICU admission, two patients (6%) had bacterial respiratory pathogens (<jats:italic>Staphylococcus<\/jats:italic> <jats:italic>aureus<\/jats:italic>, <jats:italic>Streptococcus<\/jats:italic> <jats:italic>pseudopneumoniae<\/jats:italic>) cultured in their endotracheal aspirates. Microbiome analysis recognized four potential respiratory pathogens (3 <jats:italic>Haemophilus<\/jats:italic> <jats:italic>influenza<\/jats:italic>, 1 <jats:italic>Fusobacterium necrophorum<\/jats:italic>) isolated in samples from four other patients (12%). QiaStat\u2010Dx\u00ae respiratory panel V2 detected adenovirus in one patient (3%). The prevalence of pulmonary microbial co\u2010infections is modest among COVID\u201019 patients upon admission to ICU. Microbiome analysis complements conventional microbial diagnostics in characterization of respiratory co\u2010infections.<\/jats:p>","DOI":"10.1111\/apm.13143","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T02:40:30Z","timestamp":1620268830000},"page":"431-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Extensive microbiological respiratory tract specimen characterization in critically ill COVID\u201019 patients"],"prefix":"10.1111","volume":"129","author":[{"given":"Kim","family":"Thomsen","sequence":"first","affiliation":[{"name":"The Regional Department of Clinical Microbiology Zealand University Hospital Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik Planck","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zealand University Hospital Roskilde Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Iversen","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Slagelse Hospital Slagelse Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar","family":"Wiese","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Zealand University Hospital Roskilde Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Fuursted","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi Statens Serum Institut Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik Vedel","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi Statens Serum Institut Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens J\u00f8rgen 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