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M\u00f8ller og Hustru Chastine Mc-Kinney M\u00f8llers Fond til almene Formaal","doi-asserted-by":"publisher","award":["19\u2010L\u20100283"],"award-info":[{"award-number":["19\u2010L\u20100283"]}],"id":[{"id":"10.13039\/501100005747","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Staphylococcus aureus<\/jats:italic> (SA) causes superficial and severe endovascular infections. The present <jats:italic>in vitro<\/jats:italic> study investigates the anti\u2010SA mechanisms of hyperbaric oxygen therapy (HBOT) on direct bacterial killing, antibiotic potentiation, and polymorphonuclear leukocyte (PMN) enhancement. SA was exposed to isolated human PMNs, tobramycin, ciprofloxacin, or benzylpenicillin. HBOT was used as one 90\u2010min session. Bacterial survival was evaluated after 4\u00a0h by quantitative bacteriology. PMN functionality as reactive oxygen species (ROS) production was measured by means of dihydrorhodamine 123 analysis. We showed that HBOT exhibits significant direct anti\u2010SA effects. HBOT increased the anti\u2010SA effects of PMNs by 18% after PMA stimulation (p\u00a0=\u00a00.0004) and by 15% in response to SA (p\u00a0=\u00a00.36). HBOT showed an additive effect as growth reductions of 26% to sub\u2010MICs of tobramycin (p\u00a0=\u00a00.0057), 44% to sub\u2010MICs of ciprofloxacin (p\u00a0=\u00a00.0001), and 26% to sub\u2010MICs of penicillin (p\u00a0=\u00a00.038). The present <jats:italic>in vitro<\/jats:italic> study provides evidence that HBOT has differential mechanisms mediating its anti\u2010SA effects. Our observation supports the clinical possibility for adjunctive HBOT to augment the host immune response and optimize the efficacy of antibiotic treatments.<\/jats:p>","DOI":"10.1111\/apm.13164","type":"journal-article","created":{"date-parts":[[2021,6,13]],"date-time":"2021-06-13T06:01:53Z","timestamp":1623564113000},"page":"566-573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Distinct contribution of hyperbaric oxygen therapy to human neutrophil function and antibiotic efficacy against <i>Staphylococcus aureus<\/i>"],"prefix":"10.1111","volume":"129","author":[{"given":"Franziska A.","family":"Schwartz","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen \u00d8 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian J.","family":"Lerche","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology 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