{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T14:01:12Z","timestamp":1778162472975,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,24]],"date-time":"2021-10-24T00:00:00Z","timestamp":1635033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>This study aims to investigate prevalence of <jats:italic>Mycoplasma genitalium<\/jats:italic> and macrolide resistance\u2010associated mutations and coinfection with other sexually transmitted bacteria in Southern Jutland, Denmark, where this information is very limited. Urinary samples from patients suspected of sexually transmitted bacterial infections collected at primary healthcare facilities in Southern Jutland are routinely tested for <jats:italic>Chlamydia trachomatis<\/jats:italic> and <jats:italic>Neisseria gonorrhoeae<\/jats:italic>. 601 of these samples were analysed with SpeeDx MG+23S reagents, which can detect <jats:italic>M. genitalium<\/jats:italic> and macrolide resistance\u2010mediating mutations in the 23S rRNA gene. Moreover, 147 <jats:italic>C. trachomatis<\/jats:italic> positive urinary samples from routine test were also analysed with the PCR assay to detect <jats:italic>M. genitalium<\/jats:italic>. 72 out of 601 samples were detected positive for <jats:italic>C. trachomatis<\/jats:italic> (12%), five samples (0.83%) positive for <jats:italic>N. gonorrhoeae<\/jats:italic> and 25 samples positive for <jats:italic>M. genitalium<\/jats:italic> (4.2%). 14 of the 25 <jats:italic>M. genitalium<\/jats:italic> samples were detected having 23S rRNA gene mutations associated with macrolide resistance (56%). 25 of 147 <jats:italic>C. trachomatis<\/jats:italic> positive samples were tested positive for <jats:italic>M. genitalium<\/jats:italic> (17%) and two of them were positive for <jats:italic>M. genitalium<\/jats:italic> and <jats:italic>N. gonorrhoeae<\/jats:italic> (1.4%). The high prevalence of <jats:italic>M. genitalium<\/jats:italic> and macrolide resistance\u2010associated mutation and the coinfection with <jats:italic>C. trachomatis<\/jats:italic> in the region suggesting that <jats:italic>M. genitalium<\/jats:italic> testing should be included in routine sexually transmitted infection screening.<\/jats:p>","DOI":"10.1111\/apm.13174","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T08:39:30Z","timestamp":1632818370000},"page":"706-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>Mycoplasma genitalium<\/i> prevalence and macrolide resistance\u2010associated mutations and coinfection with <i>Chlamydia trachomatis<\/i> in Southern Jutland, Denmark"],"prefix":"10.1111","volume":"129","author":[{"given":"Rasmus","family":"Desdorf","sequence":"first","affiliation":[{"name":"Focused Research Unit in Molecular Diagnostic and Clinical Research IRS\u2010Center Southern Jutland University of Southern Denmark Soenderborg Denmark"},{"name":"Department of Clinical Microbiology Hospital of Southern Jutland Soenderborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niles Moller","family":"Andersen","sequence":"additional","affiliation":[{"name":"Focused Research Unit in Molecular Diagnostic and Clinical Research IRS\u2010Center Southern Jutland University of Southern Denmark Soenderborg Denmark"},{"name":"Department of Clinical Microbiology Hospital of Southern Jutland Soenderborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Focused Research Unit in Molecular Diagnostic and Clinical Research IRS\u2010Center Southern Jutland University of Southern Denmark Soenderborg Denmark"},{"name":"Department of Clinical Microbiology Hospital of Southern Jutland Soenderborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5758387"},{"issue":"9","key":"e_1_2_9_3_1","first-page":"723","article-title":"Epididymitis: an overview","volume":"94","author":"McConaghy JR","year":"2016","journal-title":"Am Fam Physician"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13849"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000830"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30506-1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2018-053831"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix198"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2018-053823"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix145"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-052553"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2018-024175"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4513089"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.08.008"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2017-053384"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01053-16"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.09.006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2746"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2009.009143"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2016.268"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-052688"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000617"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2016.07.005"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000584"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00370-17"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01053-16"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijpvm.IJPVM_309_16"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05019-1"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156740"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3395-3"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13174","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apm.13174","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T07:08:58Z","timestamp":1693206538000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.13174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,24]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/apm.13174"],"URL":"https:\/\/doi.org\/10.1111\/apm.13174","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,24]]},"assertion":[{"value":"2020-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}