{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:56:29Z","timestamp":1762642589527},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T00:00:00Z","timestamp":1634515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF17OC0025074"],"award-info":[{"award-number":["NNF17OC0025074"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005747","name":"A.P. M\u00f8ller og Hustru Chastine Mc-Kinney M\u00f8llers Fond til almene Formaal","doi-asserted-by":"publisher","award":["19\u2010L\u20100283"],"award-info":[{"award-number":["19\u2010L\u20100283"]}],"id":[{"id":"10.13039\/501100005747","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>Infective endocarditis (IE) is a heart valve infection with high mortality rates. IE results from epithelial lesions, inducing sterile healing vegetations consisting of platelets, leucocytes, and fibrin that are susceptible for colonization by temporary bacteremia. Clinical testing of new treatments for IE is difficult and fast models sparse. The present study aimed at establishing an <jats:italic>in\u00a0vitro<\/jats:italic> vegetation simulation IE model for fast screening of novel treatment strategies. A healing promoting platelet and leucocyte\u2010rich fibrin patch was used to establish an IE organoid\u2010like model by colonization with IE\u2010associated bacterial isolates <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>Streptococcus<\/jats:italic> spp (<jats:italic>S.\u00a0mitis<\/jats:italic> group), and <jats:italic>Enterococcus faecalis<\/jats:italic>. The patch was subsequently exposed to tobramycin, ciprofloxacin, or penicillin. Bacterial colonization was evaluated by microscopy and quantitative bacteriology. We achieved stable bacterial colonization on the patch, comparable to clinical IE vegetations. Microscopy revealed uneven, biofilm\u2010like colonization of the patch. The surface\u2010associated bacteria displayed increased tolerance to antibiotics compared to planktonic bacteria. The present study succeeded in establishing an IE simulation model with the relevant pathogens <jats:italic>S.\u00a0aureus<\/jats:italic>, <jats:italic>S.\u00a0mitis<\/jats:italic> group, and <jats:italic>E<\/jats:italic>.\u00a0<jats:italic>faecalis<\/jats:italic>. The findings indicate that the IE model mirrors the natural IE process and has the potential for fast screening of treatment candidates.<\/jats:p>","DOI":"10.1111\/apm.13182","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T08:40:45Z","timestamp":1632818445000},"page":"653-662","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Novel human <i>in\u00a0vitro<\/i> vegetation simulation model for infective endocarditis"],"prefix":"10.1111","volume":"129","author":[{"given":"Franziska A.","family":"Schwartz","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Christophersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Sofie","family":"Laulund","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasmus","family":"Lundquist","sequence":"additional","affiliation":[{"name":"Reapplix Birker\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Lerche","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pia","family":"Rude Nielsen","sequence":"additional","affiliation":[{"name":"Department of Pathology Zealand University Hospital Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Bundgaard","sequence":"additional","affiliation":[{"name":"Department of Cardiology Copenhagen University Hospital Herlev Copenhagen Denmark"},{"name":"Department of Clinical Medicine University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels","family":"H\u00f8iby","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen N Denmark"},{"name":"Department of Immunology and Microbiology Costerton Biofilm Center University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Moser","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet Copenhagen N Denmark"},{"name":"Department of Immunology and Microbiology Costerton Biofilm Center University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15266-X"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2013.174"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acvd.2015.11.012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acvd.2016.02.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-017-0729-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejim.2016.05.021"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4689-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30530-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1902096"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1808312"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2012.11.006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1206782"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00069-15"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2488-5"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3392-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00315-18"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00536-10"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.7.1505"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00315-18"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1921-1924.2000"},{"key":"e_1_2_9_22_1","first-page":"1","article-title":"In vitro and in\u00a0vivo model to study bacterial adhesion to the vessel wall under flow conditions","volume":"2015","author":"Claes J","year":"2015","journal-title":"J Vis Exp"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-02-558890"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00160-18"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-020-01669-y"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000339613"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2012.00870.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1534734611426755"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12773"},{"key":"e_1_2_9_30_1","article-title":"The preparation of an autologous triple layered patch containing platelets, leukocytes and fibrin and its clinical use in the treatment of chronic","author":"Vogensen H","year":"2012","journal-title":"Wounds"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(18)30240-7"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2013.174"},{"key":"e_1_2_9_33_1","first-page":"1","article-title":"Infective endocarditis","volume":"2","author":"Holland T","year":"2017","journal-title":"Nat Rev Dis Prim"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-391858-1.00004-6"},{"key":"e_1_2_9_35_1","series-title":"Martin Dunitz medical pocket books","volume-title":"Antimicrobial chemotherapy: pocketbook","author":"Amyes S","year":"1996"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.12.015"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01208-10"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0215333"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.04.025"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.19.3.915-918.1978"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00101-18"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.5812\/cardiovascmed.18423"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2014.10.024"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02235-16"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00386-19"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx130"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.9.2034"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13182","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apm.13182","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T21:37:07Z","timestamp":1693345027000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.13182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,18]]},"references-count":46,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1111\/apm.13182"],"URL":"https:\/\/doi.org\/10.1111\/apm.13182","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,18]]},"assertion":[{"value":"2021-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}