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Infected thermal lesions can progress to chronic wounds with biofilm making them more difficult to treat. While animal models have their limitations, murine wound models are still the best tool at the moment to identify strategies to overcome these challenges. Here, we present a murine burn model, which has been developed to study biofilm formation, the significance of wound healing, and for identifying novel treatment candidates. Investigating the effect of a thermal injury in mice, we observed that 48\u2009h after introduction of the injury, the mice showed a reduction in polymorphonuclear neutrophil granulocytes (PMNs) and a reduced capacity for phagocytosis and oxidative burst. Regarding the chronic wound, <jats:italic>Pseudomonas aeruginosa<\/jats:italic> biofilm arrested wound healing and kept the wound in an inflammatory state, but suppressing PMN function by means of the PMN factor S100A8\/A9, corresponding to observations in human venous leg ulcers. Monotherapy and dual treatment with S100A8\/A9 and ciprofloxacin on <jats:italic>P.\u2009aeruginosa<\/jats:italic> biofilm\u2010infected murine wounds have been investigated. In combination, S100A8\/A9 and ciprofloxacin reduced the bacterial quantity, lowered the proinflammatory response, and increased anti\u2010inflammatory cytokines after 4\u2009days of treatment. When the treatment was prolonged, an additional prevention of resistance development was detected in all the dual\u2010treated mice. In the present review, we provide data on using the murine model for research with the aim of better understanding pathophysiology of wounds and for identifying novel treatments for humans suffering from these lesions.<\/jats:p>","DOI":"10.1111\/apm.13228","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T05:28:28Z","timestamp":1650432508000},"page":"477-490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Murine burn lesion model for studying acute and chronic wound infections"],"prefix":"10.1111","volume":"130","author":[{"given":"Henrik","family":"Calum","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Hvidovre Hospital Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Tr\u00f8strup","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery and Burns Treatment Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Sofie","family":"Laulund","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Christophersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels","family":"H\u00f8iby","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital  Copenhagen Denmark"},{"name":"Department of Immunology and Microbiology (ISIM) University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Moser","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/CWCMR.S84361"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12773"},{"key":"e_1_2_8_4_1","unstructured":"An official website of the Danish goverment: sundhed.dk. 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