{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T11:01:25Z","timestamp":1778324485944,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,22]],"date-time":"2022-05-22T00:00:00Z","timestamp":1653177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>Syndromic testing for lower respiratory tract infections with BioFire\u00ae FilmArray\u00ae Pneumonia Panel\u2009Plus (BF) detects 27 pathogens with a turn\u2010around\u2010time of one hour. We compared the performance of BF with culture. Samples from 298 hospitalized patients with suspected pneumonia routinely sent for culture were also analyzed using BF. Retrospectively, patients were clinically categorized as having \u201cpneumonia\u201d or \u201cno pneumonia.\u201d BF and culture were compared by analytical performance, which was evaluated by pathogen concordance, and by clinical performance by comparing pathogen detections in patients with and without pneumonia. The BF results for viruses and atypical bacteria were not included in the performance analysis. In 298 patient samples, BF and culture detected 285 and 142 potential pathogens, respectively. Positive percent agreement (PPA) was 88% (125\/142). In patients with community\u2010acquired pneumonia (CAP), clinical sensitivity was 70% and 51%, and specificity was 43% and 71% for BF and culture, respectively. In patients with hospital\u2010acquired pneumonia, the corresponding numbers were 55% and 23%, and 47% and 68%. There was no significant improvement of performance, when only high\u2010quality sputum samples were considered. Efficacy of both BF and culture was low. Both tests are best used in CAP patients for whom the diagnosis has already been clinically established. Indiscriminate use may be clinically misleading and a cause of improper use of antibiotics.<\/jats:p>","DOI":"10.1111\/apm.13232","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T12:49:52Z","timestamp":1651495792000},"page":"417-426","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Performance of <scp>PCR<\/scp>\u2010based syndromic testing compared to bacterial culture in patients with suspected pneumonia applying microscopy for quality assessment"],"prefix":"10.1111","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4579-9402","authenticated-orcid":false,"given":"Vigith","family":"Andrews","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital \u2013 Amager and Hvidovre  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette","family":"Pinholt","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital \u2013 Amager and Hvidovre  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uffe Vest","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital \u2013 Amager and Hvidovre  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristian","family":"Sch\u00f8nning","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital \u2013 Rigshospitalet  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lillian Marie","family":"S\u00f8es","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital \u2013 Amager and Hvidovre  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gorm","family":"Lisby","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital \u2013 Amager and Hvidovre  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1183\/1025448x.erm6314","volume-title":"Community\u2010Acquired Pneumonia","author":"Chalmers JD","year":"2014"},{"key":"e_1_2_8_3_1","volume-title":"The top 10 causes of death [internet]","author":"World Health Organization","year":"2014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw353"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/511159"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1500245"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0115"},{"key":"e_1_2_8_8_1","volume-title":"Pneumonia in adults: diagnosis and management. 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