{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:44:35Z","timestamp":1790289875308,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>\n                    Methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) is resistant to almost all \u03b2\u2010lactam antibiotics. Hence, new ways to control MRSA infection, such as antibacterial antibodies, need to be explored. \u03b1\u2010hemolysin is the most important virulence factor widely expressed in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . This study aimed to develop a new fully human antibody against \u03b1\u2010hemolysin of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and research its neutralizing effect\n                    <jats:italic>.<\/jats:italic>\n                    The single\u2010chain antibody fragments (scFvs) against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    were screened from a fully human scFv library using phage display technology. The selected scFvs had good binding affinities to \u03b1\u2010hemolysin and\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . The IgG\u2010like scFv\u2010Fc inserted into the pcDNA3.1 or pMH3 vector was expressed in HEK293F suspension cells to extend the half\u2010life and restore Fc function. The size of purified scFv\u2010Fc was about 55\u2009kDa. The functions of expressed scFv\u2010Fcs against \u03b1\u2010hemolysin were validated. The cytotoxicity assays showed that scFv555\u2010Fc had better protective effects on A549 cells than other scFv\u2010Fcs. The results of anti\u2010rabbit erythrocyte lysis and A549 cell apoptosis assay confirmed that scFv555\u2010Fc had a significant neutralizing effect on \u03b1\u2010hemolysin. The scFv555\u2010Fc was used to construct the docking model of antigen\u2013antibody complexes using Discovery Studio software. It predicted that the key binding sites of \u03b1\u2010hemolysin were TYR28, LYS37, PHE39, ARG56, and LYS58, which might be the key toxic sites of \u03b1\u2010hemolysin. A novel fully human scFv\u2010Fc antibody neutralizing the \u03b1\u2010hemolysin toxin of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    was successfully developed. The findings might provide a new theoretical basis and treatment method for preventing MRSA infection.\n                  <\/jats:p>","DOI":"10.1111\/apm.13258","type":"journal-article","created":{"date-parts":[[2022,6,25]],"date-time":"2022-06-25T05:38:21Z","timestamp":1656135501000},"page":"578-589","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A novel fully human recombinant antibody neutralizing \u03b1\u2010hemolysin of\n                    <i>Staphylococcus aureus<\/i>"],"prefix":"10.1111","volume":"130","author":[{"given":"Wenfeng","family":"Xu","sequence":"first","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Peng","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Li","sequence":"additional","affiliation":[{"name":"Clinical Pharmacy &amp; GCP Center the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wu","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Chen","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yu","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Ye","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siji","family":"Nian","sequence":"additional","affiliation":[{"name":"Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808203390806"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.33"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/345476"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00042-09"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2012.682573"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins4060430"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S47305"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp201"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1275535"},{"issue":"1","key":"e_1_2_11_11_1","first-page":"e01020\u201016","article-title":"Safety, tolerability, and pharmacokinetics of MEDI4893, an investigational, extended\u2010half\u2010life, anti\u2010Staphylococcus aureus alpha\u2010toxin human monoclonal antibody","volume":"61","author":"Yu X","year":"2016","journal-title":"Healthy Adults Clin Trial"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2016.07.004"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1086\/590157"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.04.003"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075103"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02456-16"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03918-14"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9853-1_8"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-016-0146-z"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.1.7821762"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867324666170113112759"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2016.03.005"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4632-9"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2016.1212149"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2005.01.001"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05589-11"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001815107"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.02.008"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.601328"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2013.01.008"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13258","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apm.13258","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T15:49:58Z","timestamp":1692805798000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.13258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,12]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1111\/apm.13258"],"URL":"https:\/\/doi.org\/10.1111\/apm.13258","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,12]]},"assertion":[{"value":"2022-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}