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We included 71 OTSCC patients treated with surgery combined with RT or CRT. We evaluated the association between tumor budding, tumor\u2013stroma ratio (TSR), depth of invasion (DOI), tumor\u2010infiltrating lymphocytes (TILs), hypoxia\u2010inducible factor\u20101alpha (HIF\u20101alpha) expression, octamer\u2010binding transcription factor 4 (OCT4) expression, high\u2010endothelial venules (HEVs), and disease\u2010free survival (DFS) using uni\u2010 and multivariate analyses. No significant association was observed between the different histological and molecular markers (TSR, DOI, TILs, HEV, HIF\u20101alph, OCT4) and DFS. However, an associative trend between DOI, budding, and DFS was noted. Further studies with larger cohorts are needed to explore the prognostic value of DOI and budding for OTSCC patients treated with postoperative RT or CRT.<\/jats:p>","DOI":"10.1111\/apm.13298","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T14:16:51Z","timestamp":1674656211000},"page":"142-151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prognostic histological markers in oral tongue squamous cell carcinoma patients treated with (chemo)radiotherapy"],"prefix":"10.1111","volume":"131","author":[{"given":"Aini","family":"Hyyti\u00e4inen","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases University of Helsinki  Helsinki Finland"},{"name":"Translational Immunology Research Program, Faculty of Medicine University of Helsinki  Helsinki 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