{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T11:01:43Z","timestamp":1792839703372,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>The introduction of direct\u2010acting antiviral (DAA) treatment of hepatitis C virus (HCV) infected patients has greatly increased treatment success rates. However, viral response kinetics to DAA treatment may depend on pre\u2010existing resistance\u2010associated substitutions (RASs) in HCV. The aim of this study was to describe how pre\u2010existing RASs affect DAA treatment\u2010induced reduction in HCV RNA titers in HCV genotypes 1\u2010 and 3\u2010infected individuals. Patients with HCV genotype 1 infection (N\u2009=\u200931) treated with either sofosbuvir\/ledipasvir\/ribavirin or paritaprevir\/ombitasvir\/ritonavir\/dasabuvir\/ribavirin and HCV genotype 3\u2010infected patients (N\u2009=\u200916) treated with either sofosbuvir\/daclatasvir\/ribavirin or sofosbuvir\/ribavirin were analyzed. HCV RNA levels were determined at baseline and frequently during treatment, and RAS profiles were obtained by deep sequencing at baseline. In total, 33\/47 (70.2%) of the patients had baseline RASs. However, treatment\u2010specific RASs were detected at baseline only in 12.9% and 18.8% of HCV genotypes 1\u2010 and 3\u2010infected patients, respectively. In genotype 1\u2010infected individuals, reduction in HCV RNA titer during the first week of treatment was not affected by evidence of either treatment\u2010specific RASs or cirrhosis or treatment regimen. In genotype 3\u2010infected individuals receiving sofosbuvir\/daclatasvir\/ribavirin, the presence of daclatasvir\u2010specific NS5A RASs at baseline correlated with a reduced decline of HCV RNA in the first treatment week. For both genotypes 1\u2010 and 3\u2010infected individuals, cirrhosis but not treatment\u2010specific RAS were associated with the time of clearance of HCV RNA. It is, however, important to note that this study involves DAA regimens that were used only during the original introduction of interferon\u2010free DAA\u2010based treatments.<\/jats:p>","DOI":"10.1111\/apm.13335","type":"journal-article","created":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T09:29:35Z","timestamp":1687685375000},"page":"426-433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Pre\u2010existing, treatment\u2010specific resistance\u2010associated substitutions in hepatitis C virus genotype 1 and 3 and viral <scp>RNA<\/scp> titers during treatment with direct\u2010acting antivirals"],"prefix":"10.1111","volume":"131","author":[{"given":"Christina","family":"S\u00f8lund","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Copenhagen University Hospital  Hvidovre Denmark"},{"name":"Copenhagen Hepatitis C Program (CO\u2010HEP), Department of Infectious Diseases Copenhagen 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