{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:53:59Z","timestamp":1762325639027,"version":"3.28.0"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV 19\u20100519"],"award-info":[{"award-number":["APVV 19\u20100519"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>A total of, 78<jats:italic>Clostridium septicum<\/jats:italic>(CLSE) isolates were screened for genes encoding: \u03b1\u2010toxin, flagellin, and resistance to vancomycin (<jats:italic>VANg<\/jats:italic>). The isolates were also tested for their ability to form biofilm and their antibiotic susceptibility. All isolates were positive for \u03b1\u2010toxin and flagellin genes. However, only 19 isolates (24.3%) showed prevalence for<jats:italic>VANg<\/jats:italic>. We observed the strongest capacity to form a biofilm (100%) in isolates from patients with oncologic or septic and febrile diagnoses. This percentage was also very high in patients with colitis and gastrointestinal hemorrhage (72.7%). No less than 43 isolates showed antibiotic resistance, and 21 were multidrug\u2010resistant (MDR). Interestingly, our studies showed a correlation between antibiotic resistance and biofilm formation. A statistically significant difference was observed between biofilm\u2010forming MDR isolates and those with low\/no biofilm\u2010forming ability. However, the most impressive observation was the correlation with mortality rate. While the overall mortality rate for CLSE infections was 16.7% (13\/78), the mortality rate for patients infected with MDR isolates forming biofilm moderately or strongly reached 38.1% (8\/21). This number increased even further when only infections with the biofilm\u2010forming<jats:italic>VANg<\/jats:italic>\u2010positive isolates were considered (61.5%; 8\/13). Therefore, the ability of a<jats:italic>VANg<\/jats:italic>\u2010positive CLSE isolate to form a biofilm has been suggested as a biomarker of poor prognosis.<\/jats:p>","DOI":"10.1111\/apm.13338","type":"journal-article","created":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T09:27:53Z","timestamp":1687685273000},"page":"434-441","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antibiotic resistance and biofilm\u2010forming ability of \u03b1\u2010toxin\u2010positive<i>Clostridium septicum<\/i>isolates worsen patient prognosis"],"prefix":"10.1111","volume":"131","author":[{"given":"Jozef","family":"Kuzma","sequence":"first","affiliation":[{"name":"Biomedical Research Center of Slovak Academy of Sciences Bratislava Slovakia"},{"name":"Faculty of Medicine, Institute of Laboratory Medicine University of Ostrava Ostrava Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sof\u00eda 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