{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:27:05Z","timestamp":1791419225603,"version":"4.3.3"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:00:00Z","timestamp":1687219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>\n                    <jats:italic>Candida spp.<\/jats:italic>\n                    are opportunistic yeasts capable of forming biofilms, which contribute to resistance, increasing the urgency for new effective antifungal therapies. Repurposing existing drugs could significantly accelerate the development of novel therapies against candidiasis. We screened the Pandemic Response Box containing 400 diverse drug\u2010like molecules active against bacteria, viruses or fungi, for inhibitors of\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and\n                    <jats:italic>Candida auris<\/jats:italic>\n                    biofilm formation. Initial hits were identified based on the demonstration of &gt;70% inhibitory activity. Dose\u2013response assays were used to confirm the antifungal activity of initial hits and establish their potency. The spectrum of antifungal activity of the leading compounds was determined against a panel of medically important fungi, and the\n                    <jats:italic>in vivo<\/jats:italic>\n                    activity of the leading repositionable agent was evaluated in murine models of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    and\n                    <jats:italic>C. auris<\/jats:italic>\n                    systemic candidiasis. The primary screening identified 20 hit compounds, and their antifungal activity and potency against\n                    <jats:italic>C.\u2009albicans<\/jats:italic>\n                    and\n                    <jats:italic>C. auris<\/jats:italic>\n                    were validated using dose\u2013response measurements. From these experiments, the rapalog everolimus, emerged as the leading repositionable candidate. Everolimus displayed potent antifungal activity against different\n                    <jats:italic>Candida spp.<\/jats:italic>\n                    , but more moderate levels of activity against filamentous fungi. Treatment with everolimus increased survival of mice infected with\n                    <jats:italic>C. albicans<\/jats:italic>\n                    , but not those with\n                    <jats:italic>C. auris<\/jats:italic>\n                    . The screening of the Pandemic Response Box resulted in the identification of several drugs with novel antifungal activity, with everolimus emerging as the main repositionable candidate. Further\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies are needed to confirm its potential therapeutic use.\n                  <\/jats:p>","DOI":"10.1111\/apm.13342","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T04:59:17Z","timestamp":1687237157000},"page":"613-625","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Screening the medicine for malaria venture's Pandemic Response Box to identify novel inhibitors of\n                    <i>Candida albicans<\/i>\n                    and\n                    <i>Candida auris<\/i>\n                    biofilm formation"],"prefix":"10.1111","volume":"131","author":[{"given":"Olabayo H.","family":"Ajetunmobi","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology &amp; Immunology, and South Texas Center for Emerging Infectious Diseases The University of Texas at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok K.","family":"Chaturvedi","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology &amp; Immunology, and South Texas Center for Emerging Infectious Diseases The University of Texas at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Badali","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology &amp; Immunology, and South Texas Center for Emerging Infectious Diseases The University of Texas at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Vaccaro","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology &amp; Immunology, and South Texas Center for Emerging Infectious Diseases The University of Texas at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Najvar","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Infectious Diseases The University of Texas Health Science Center at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Floyd L.","family":"Wormley","sequence":"additional","affiliation":[{"name":"Department of Biology Texas Christian University  Fort Worth Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan P.","family":"Wiederhold","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine The University of Texas Health Science Center at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F.","family":"Patterson","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Infectious Diseases The University of Texas Health Science Center at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Lopez\u2010Ribot","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology &amp; 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