{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T16:10:33Z","timestamp":1734711033823,"version":"3.32.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>Bacterial aerobic respiration may determine the outcome of antibiotic treatment in experimental settings, but the clinical relevance of bacterial aerobic respiration for the outcome of antibiotic treatment has not been tested. Therefore, we hypothesized that bacterial aerobic respiration is higher in sputum from patients with acute lower respiratory tract infections (aLRTI), than in sputum from patients with chronic LRTI (cLRTI), where the bacteria persist despite antibiotic treatment. The bacterial aerobic respiration was determined according to the dynamics of the oxygen (O<jats:sub>2<\/jats:sub>) concentration in sputum from aLRTI patients (<jats:italic>n<\/jats:italic>\u2009=\u200952). This result was evaluated by comparison to previously published data from patients with cLRTI. O<jats:sub>2<\/jats:sub> consumption resulting in anoxic zones was more frequent in sputum with detected bacterial pathogens. The bacterial aerobic respiration in aLRTI sputum approximated 55% of the total O<jats:sub>2<\/jats:sub> consumption, which was significantly higher than previously published for cLRTI. The bacterial aerobic respiration in sputum was higher in aLRTI patients than previously seen in cLRTI patients, indicating the presence of bacteria with a sensitive physiology in aLRTI. These variations in bacterial physiology between aLRTI patients and cLRTI patients may contribute the huge difference in treatment success between the two patient groups.<\/jats:p>","DOI":"10.1111\/apm.13381","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T11:28:31Z","timestamp":1706527711000},"page":"1078-1085","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bacterial aerobic respiration is a major consumer of oxygen in sputum from patients with acute lower respiratory tract infection"],"prefix":"10.1111","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1648-7186","authenticated-orcid":false,"given":"Peter \u00d8strup","family":"Jensen","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet  Copenhagen Denmark"},{"name":"Costerton Biofilm Center, Institute of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"},{"name":"Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Copenhagen University Hospital Rigshospitalet  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernille","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnold Matovu","family":"Dungu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Infectious Diseases Copenhagen University Hospital \u2013 North Zealand  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gertrud Baunb\u00e6k","family":"Egelund","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Infectious Diseases Copenhagen University Hospital \u2013 North Zealand  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas Vestergaard","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Infectious Diseases Copenhagen University Hospital \u2013 North Zealand  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernille","family":"Ravn","sequence":"additional","affiliation":[{"name":"Department of Medicine Section for Infectious Diseases Herlev\u2010 Gentofte University Hospital  Hellerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgitte","family":"Lindegaard","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Infectious Diseases Copenhagen University Hospital \u2013 North Zealand  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederik Bo\u00ebtius","family":"Hertz","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Herlev Hospital  Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Bjarnsholt","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet  Copenhagen Denmark"},{"name":"Costerton Biofilm Center, Institute of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Faurholt\u2010Jepsen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Rigshospitalet  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette","family":"Kolpen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Rigshospitalet  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.38993.560984.BE"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2012.10.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.09.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2013.06.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2021-217576"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009112"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12668"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/27.2.177"},{"issue":"5","key":"e_1_2_9_10_1","first-page":"1297","article-title":"The rate of killing of Escherichia coli by beta\u2010lactam antibiotics is strictly proportional to the rate of bacterial growth","volume":"132","author":"Tuomanen E","year":"1986","journal-title":"J Gen Microbiol"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00615"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2012.10.002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509743112"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-10992-8_9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.114512"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2019.01.005"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198909"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/br.40.3.652-680.1976"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00122-5"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0213870"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084353"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00767-15"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0536-0"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2208035119"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.09.059"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-food-022811-101255"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2011.06.016"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00103"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01882-07"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.625597"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3148"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2022.102197"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010115"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1500199"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27463-0"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T15:30:23Z","timestamp":1734708623000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.13381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":34,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/apm.13381"],"URL":"https:\/\/doi.org\/10.1111\/apm.13381","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"type":"print","value":"0903-4641"},{"type":"electronic","value":"1600-0463"}],"subject":[],"published":{"date-parts":[[2024,1,29]]},"assertion":[{"value":"2023-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}