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Samples were stored at \u201370\u2009\u00b0C until being analysed with multiplex PCR for seven respiratory bacteria. Of the 912 patients enrolled, those with positive bacterial samples (n\u2009=\u2009130, 14%) were significantly younger than those with a negative finding (55.5\u2009years vs 62.2\u2009years, p\u2009&lt;\u20090.001), and their mean C\u2010reactive protein (CRP) concentration was higher (110\u2009mg\/L vs 59\u2009mg\/L, p\u2009&lt;\u20090.0001). Patients with a positive respiratory bacterial finding had a higher probability of pneumonia (35% vs 13%, p\u2009&lt;\u20090.001) and a higher likelihood of receiving a prescription for antibiotics than those with a negative finding (79% vs 59%, p\u2009&lt;\u20090.0001). Positive detection of <jats:italic>Streptococcus pneumoniae<\/jats:italic> was associated with a 4.5\u2010fold risk of pneumonia in a multivariate model and detection of an atypical respiratory pathogen with a 9\u2010fold risk. Bacterial PCR performed on nasopharyngeal samples appeared to offer a valuable addition to the diagnostics of infections in adults in acute care.<\/jats:p>","DOI":"10.1111\/apm.13403","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:30:38Z","timestamp":1710408638000},"page":"444-451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multiplex PCR for respiratory bacteria in acute care"],"prefix":"10.1111","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3093-0262","authenticated-orcid":false,"given":"Elina","family":"Saarela","sequence":"first","affiliation":[{"name":"Research Unit of Biomedicine and Internal Medicine University of Oulu and Medical Research Center Oulu, Oulu University Hospital  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjo","family":"Renko","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Eastern Finland and Kuopio University Hospital  Kuopio Finland"},{"name":"Research Unit of Clinical Medicine University of Oulu  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matti","family":"Uhari","sequence":"additional","affiliation":[{"name":"Research Unit of Clinical Medicine University of Oulu  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tytti","family":"Pokka","sequence":"additional","affiliation":[{"name":"Research Unit of Clinical Medicine University of Oulu  Oulu Finland"},{"name":"Research Service Unit Oulu University Hospital  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heikki","family":"Kauma","sequence":"additional","affiliation":[{"name":"Research Unit of Biomedicine and Internal Medicine University of Oulu and Medical Research Center Oulu, Oulu University Hospital  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terhi S.","family":"Ruuska","sequence":"additional","affiliation":[{"name":"Research Unit of Clinical Medicine University of Oulu  Oulu Finland"},{"name":"Department of Pediatrics and Adolescent Medicine Oulu University Hospital  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt301"},{"key":"e_1_2_12_3_1","unstructured":"WHO.The top 10 causes of death. 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