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Antibodies against Melan\u2010A are thus used for identifying melanocytic tumors, but some Melan\u2010A antibodies show an additional \u2013 diagnostically useful \u2013 cross\u2010reactivity against an unspecified protein involved in corticosteroid hormone synthesis. To comprehensively compare the staining patterns of a specific and a cross\u2010reactive Melan\u2010A antibody in normal and neoplastic tissues, tissue microarrays containing 15,840 samples from 133 different tumor types and subtypes as well as 608 samples of 76 different normal tissue types were analyzed by immunohistochemistry. For the Melan\u2010A\u2010specific antibody \u2018Melan\u2010A specific\u2019 (MSVA\u2010900M), Melan\u2010A positivity was seen in 96.0% of 25 benign nevi, 93.0% of 40 primary and 86.7% of 75 metastatic melanomas, 82.4% of 85 renal angiomyolipomas as well as 96.4% of 84 neurofibromas, 2.2% of 46 granular cell tumors, 1.0% of 104 schwannomas, and 1.1% of 87 leiomyosarcomas. The cross\u2010reactive antibody \u2018Melan\u2010A+' (MSVA\u2010901M+) stained 98.1% of the tumors stained by \u2018Melan\u2010A specific\u2019. In addition, high positivity rates were seen in sex\u2010cord\u2010stroma tumors of the ovary (35.3%\u2013100%) and the testis (86.7%) as well as for adrenocortical neoplasms (76.3%\u201383.0%). Only nine further tumor groups showed Melan\u2010A+ staining, including five different categories of urothelial carcinomas. Our data provide a comprehensive overview on the staining patterns of specific and cross\u2010reactive Melan\u2010A antibodies. The data demonstrate that both antibodies are highly useful for their specific purpose. 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