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PIM2 is a well\u2010known oncogene and is regulated by cell signaling pathways like the JAK\/STAT\u2010 and NF\u2010kB\u2010pathway, key regulators in the pathogenesis of CTCL. The aim of this study was to examine the role of PIM2 in MF. PIM2 gene expression was measured in 81 formalin\u2010fixed paraffin\u2010embedded skin biopsies from patients with MF and 46 control biopsies from healthy skin (HS) and benign inflammatory skin disease (BID). Validation of PIM2 protein expression was performed on selected biopsies with immunohistochemical staining. We found a significant difference in gene expression levels between both early stage MF and HS (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001), and BID (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). In addition, the PIM2 gene expression was higher in advanced\u2010stage MF compared to early stage disease (<jats:italic>p<\/jats:italic>\u2009=\u20090.0001). No significant difference in gene expression levels was found between patients with and without disease progression. In conclusion, we found PIM2 expression is significantly increased in MF compared to controls, and in advanced\u2010stage MF compared to early stage MF. These findings could potentially have diagnostic value in discriminating early stage MF from BID.<\/jats:p>","DOI":"10.1111\/apm.13423","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T03:28:11Z","timestamp":1715916491000},"page":"564-570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stage\u2010related increase in <scp>PIM2<\/scp> expression in mycosis\u00a0fungoides"],"prefix":"10.1111","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9177-7841","authenticated-orcid":false,"given":"Mie Holm","family":"Nielsen","sequence":"first","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital \u2013 Zealand University Hospital Roskilde  Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pia Rude","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital \u2013 Zealand University Hospital Roskilde  Roskilde Denmark"},{"name":"Department of Immunology and Microbiology, LEO Foundation Skin Immunology Research Center University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Bzorek","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital \u2013 Zealand University Hospital Roskilde  Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens Ole","family":"Eriksen","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital \u2013 Zealand University Hospital Roskilde  Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Wehkamp","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital  Kiel Schleswig\u2010Holstein 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