{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:45:23Z","timestamp":1789753523914,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T00:00:00Z","timestamp":1717545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> infection causes pneumonia and sepsis. Previous research found that X\u2010ray radiation can induce <jats:italic>P.\u2009aeruginosa<\/jats:italic> to release outer membrane vesicles (OMVs) of relatively consistent sizes. This study found that OMVs derived from X\u2010ray\u2010irradiated <jats:italic>P.\u2009aeruginosa<\/jats:italic> can significantly inhibit lung leakage, inflammatory cell infiltrating into lung, and the production of pro\u2010inflammatory cytokines, IL\u20101\u03b2 and TNF\u03b1 caused by <jats:italic>P.\u2009aeruginosa<\/jats:italic> infection under preventive and therapeutic administration conditions. Under the same conditions, OMVs also significantly alleviated pathological characteristics of lung injury, including pulmonary edema, pulmonary hemorrhage, and alveolar wall thickening. OMVs also significantly reduced bacterial burdens in peritoneal cavity, accompanied by a reduction in the number of viable bacteria capable of forming bacterial colonies. Pretreating macrophages and neutrophils with OMVs enhances their bactericidal ability. When bacteria were cocultured with treated cells, the number of viable bacteria capable of forming bacterial colonies was significantly reduced. OMVs themselves have not been shown to cause any lung injury or affect bacterial viability. Therefore, OMVs derived from X\u2010ray\u2010irradiated <jats:italic>P.\u2009aeruginosa<\/jats:italic> may not only be applied in prevention and treatment of diseases associated with <jats:italic>P.\u2009aeruginosa<\/jats:italic> infection, but also served as an excellent vaccine development platform.<\/jats:p>","DOI":"10.1111\/apm.13444","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T07:41:27Z","timestamp":1717573287000},"page":"646-656","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Outer membrane vesicles from X\u2010ray\u2010irradiated <i>Pseudomonas aeruginosa<\/i> alleviate lung injury caused by <i>P.\u2009aeruginosa<\/i> infection\u2010mediated sepsis"],"prefix":"10.1111","volume":"132","author":[{"given":"Hongxia","family":"Bi","sequence":"first","affiliation":[{"name":"Center of Infectious Disease, West China Hospital Sichuan University  Chengdu 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