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Infections can hinder wound healing posing challenges. L\u2010PRF's potential for regeneration and antimicrobial action has been studied. Considering the increasing concern about antibiotic resistance, assessing the antimicrobial properties of L\u2010PRF provides valuable insights into its potential as a therapeutic agent in postoperative infections. Twenty volunteers were enrolled in the study, following ethical guidelines, and obtaining informed consent. Blood samples were collected and L\u2010PRF was prepared. Microbial suspensions were prepared, and susceptibility testing was performed using the Kirby\u2010Bauer agar diffusion method. The study revealed significant heterogeneity in the susceptibility to L\u2010PRF. All L\u2010PRF membrane samples exhibited antimicrobial activity against <jats:italic>P. aeruginosa<\/jats:italic>, with inhibition zones of 13\u2009mm\u2009\u00b1\u20093.85 SD. <jats:italic>Enterococcus faecalis<\/jats:italic> displayed inhibition diameter of 7.25\u2009mm\u2009\u00b1\u20095.15 SD. <jats:italic>Candida albicans<\/jats:italic> susceptibility to L\u2010PRF varied among samples, with both inhibitory and non\u2010inhibitory results. Results showed varying degrees of antimicrobial activity, particularly against <jats:italic>P. aeruginosa,<\/jats:italic> and highlight the complexity of the L\u2010PRF\u2010microorganism interaction. Further investigations are needed to elucidate the clinical implications and optimize the use of L\u2010PRF.<\/jats:p>","DOI":"10.1111\/apm.13468","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T06:59:14Z","timestamp":1726729154000},"page":"859-868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the antimicrobial potential of Leukocyte\u2010 and Platelet\u2010Rich Fibrin \u2013 an <i>in vitro<\/i> study"],"prefix":"10.1111","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0289-8064","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Melo\u2010Ferraz","sequence":"first","affiliation":[{"name":"UNIPRO \u2013 Oral Pathology and Rehabilitation Research Unit University Institute of Health Sciences (IUCS), CESPU, CRL  Gandra 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