{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:41:37Z","timestamp":1747183297994,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T00:00:00Z","timestamp":1726617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF21OC0072708"],"award-info":[{"award-number":["NNF21OC0072708"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013814","name":"Klinisk Institut, Syddansk Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013814","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>The purpose of this study was to establish a porcine model of urinary tract infection (UTI) with gram\u2010positive uropathogens. Ten female domestic pigs were experimentally inoculated with human UTI isolates of <jats:italic>Enterococcus faecalis<\/jats:italic> (n\u2009=\u20093), <jats:italic>Staphylococcus saprophyticus<\/jats:italic> (n\u2009=\u20093), or S<jats:italic>taphylococcus aureus<\/jats:italic> (n\u2009=\u20094) and followed with regular urine samples. Bladders and kidneys were aseptically removed at termination (5\u20137\u2009days post infection) and assessed by gross pathology and bacterial enumeration. <jats:italic>Enterococcus faecalis<\/jats:italic> (n\u2009=\u20093 of 3) and <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> (n\u2009=\u20092 of 4) successfully colonized the pig bladders. Inoculation with <jats:italic>S<\/jats:italic>. <jats:italic>saprophyticus<\/jats:italic> never resulted in detectable bacteriuria. All infected pigs had cleared the infection spontaneously before termination. Surprisingly, three (of four) pigs inoculated with <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> led to spontaneous infection with opportunistic pathogens. Also, one pig colonized with <jats:italic>E<\/jats:italic>. <jats:italic>faecalis<\/jats:italic> resulted in spontaneous infection with <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>. In conlusion, the pig supports experimental UTI with <jats:italic>E<\/jats:italic>. <jats:italic>faecalis<\/jats:italic> for up to 24\u2009h but not prolonged infection. <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> and <jats:italic>S<\/jats:italic>. <jats:italic>saprophyticus<\/jats:italic> fails to cause UTI in pigs and other animals should be considered for studying these pathogens.<\/jats:p>","DOI":"10.1111\/apm.13469","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T06:59:14Z","timestamp":1726729154000},"page":"807-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The infectious capacity of <i>Enterococcus faecalis<\/i>, <i>Staphylococcus aureus<\/i>, and <i>Staphylococcus saprophyticus<\/i> in a porcine model of urinary tract 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