{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:52:31Z","timestamp":1791582751935,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:00:00Z","timestamp":1726790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005111","name":"Rigshospitalet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>The rise in osteomyelitis and periprosthetic joint infections, in combination with increasing life expectancy and the prevalence of diabetes, underscores the urgent need for rapid and accurate diagnostic tools. Conventional culture\u2010based methods are often time\u2010consuming and prone to false\u2010negatives, leading to prolonged and inappropriate antibiotic treatments. This study aims to improve osteomyelitis diagnostics by decreasing the time to detection and the time to an antibiotic susceptibility result to enable a targeted treatment using isothermal microcalorimetry (IMC). IMC measures heat flow in real\u2010time, providing insights into bacterial metabolism without the need for labeling. Using clinical isolates from bone infections, assessing their response to antibiotics through IMC, we demonstrated that IMC could detect bacteria within 4\u2009h and determine antimicrobial susceptibility profiles within 2\u201322\u2009h (median 4.85, range 1.28\u201321.78). This is significantly faster than traditional methods. A decision tree, based on antibiotic susceptibility, accurately categorized pathogens, achieving high accuracy (74\u2013100%), sensitivity (100%), and specificity (65\u2013100%). These findings suggest that IMC could redefine diagnostics of bone and joint infections and potentially infections in general, offering timely and precise treatment guidance, thereby improving patient outcomes and reducing health care burdens. Further optimization and clinical validation are needed to fully integrate IMC into routine diagnostics.<\/jats:p>","DOI":"10.1111\/apm.13470","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T11:34:58Z","timestamp":1726832098000},"page":"814-823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antimicrobial susceptibility testing of bone and joint pathogens using isothermal microcalorimetry"],"prefix":"10.1111","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5014-346X","authenticated-orcid":false,"given":"Mads H.","family":"Christensen","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, Costerton Biofilm Center University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1671-2155","authenticated-orcid":false,"given":"Tim H.","family":"Jakobsen","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, Costerton Biofilm Center University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0675-4554","authenticated-orcid":false,"given":"Mads","family":"Lichtenberg","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, Costerton Biofilm Center University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederik B.","family":"Hertz","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Copenhagen University Hospital, Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benny","family":"Dahl","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Surgery Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bjarnsholt","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, Costerton Biofilm Center University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Clinical Microbiology Copenhagen University Hospital, Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00686-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1302\/2058-5241.4.180092"},{"key":"e_1_2_6_4_1","first-page":"459","article-title":"Projections of hip arthroplasty in OECD countries up to 2050","volume":"28","author":"Christof Pabinger HL","year":"2017","journal-title":"Hip Int"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/CORR.0000000000001838"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16727-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3810\/psm.2008.12.11"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1302\/2058-5241.4.180067"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/CORR.0000000000002186"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compositesb.2022.110428"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17060855"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/s101009369"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2011.10902.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-020-03366-3"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","unstructured":"Mads LichtenbergKNK BlaineF JuliusB\u2010K.Thomas Bjarnsholt. Metabolic flux fingerprinting differentiates planktonic and biofilm states of Pseudomonas aeruginosa and Staphylococcus aureus.bioRxiv.2020.","DOI":"10.1101\/2020.07.15.203828"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111515"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.06.009"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.01.026"},{"key":"e_1_2_6_19_1","unstructured":"The European Committee on Antimicrobial Susceptibility Testing.Breakpoint tables for interpretation of MICs and zone diameters. 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