{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T18:27:24Z","timestamp":1754159244203,"version":"3.41.2"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>Pleomorphic adenoma (PA) is a benign salivary gland tumour that may recur or undergo malignant transformation (CXPA). Toll\u2010like receptors (TLR) mediate immune responses triggered by various agents such as viruses and are related to tumour formation either by stimulating or suppressing their growth, with variation across different tumour entities. We compared TLR immunohistochemical expression in PA, its recurrent counterparts and CXPA and evaluated the effect of virus presence in these tumours. We studied the expression of TLR\u20102, \u20103, \u20105, \u20107 and \u20109 in 25 PA, 34 recurrent PA and 15 CXPA tumour samples. In addition, we examined the TLR expression levels in the presence and absence of herpes\u2010, polyoma\u2010\u00a0and parvovirus DNA in a subset of tumours (n\u2009=\u200920). CXPA expressed significantly more TLR\u20105 and TLR\u20109 in the nucleus, cytoplasm and cell membrane compared with benign PA. The presence of virus DNA did not notably affect the TLR expression. TLR expression patterns seem to reflect tumour behaviour but are independent of the presence of viruses tested in this study.<\/jats:p>","DOI":"10.1111\/apm.13505","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T05:09:50Z","timestamp":1734325790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of toll\u2010like receptors in recurrent pleomorphic adenoma and carcinoma ex pleomorphic adenoma"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2310-039X","authenticated-orcid":false,"given":"Maria K.","family":"Jauhiainen","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Helsinki University Hospital and University of Helsinki  Helsinki Finland"},{"name":"Department of Virology University of Helsinki  Helsinki Finland"},{"name":"Research Program in Systems Oncology, Faculty of Medicine University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4697-6538","authenticated-orcid":false,"given":"Anttoni","family":"Markkanen","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Helsinki and Turku  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6560-1128","authenticated-orcid":false,"given":"Timo","family":"Atula","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8103-5237","authenticated-orcid":false,"given":"Katri","family":"Aro","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0456-4965","authenticated-orcid":false,"given":"Caj","family":"Haglund","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Helsinki and Turku  Helsinki Finland"},{"name":"Translational Cancer Medicine, Research Programs Unit, Faculty of Medicine University of Helsinki  Helsinki Finland"},{"name":"Department of Surgery Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4582-8317","authenticated-orcid":false,"given":"Maria","family":"S\u00f6derlund\u2010Venermo","sequence":"additional","affiliation":[{"name":"Department of Virology University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0451-2404","authenticated-orcid":false,"given":"Antti A.","family":"M\u00e4kitie","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Helsinki University Hospital and University of Helsinki  Helsinki Finland"},{"name":"Research Program in Systems Oncology, Faculty of Medicine University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6601-4216","authenticated-orcid":false,"given":"Saku T.","family":"Sinkkonen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology \u2013 Head and Neck Surgery Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6079-7881","authenticated-orcid":false,"given":"Jaana","family":"Hagstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Helsinki and Turku  Helsinki Finland"},{"name":"Translational Cancer Medicine, Research Programs Unit, Faculty of Medicine University of Helsinki  Helsinki Finland"},{"name":"Department of Oral Pathology and Radiology University of Turku  Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.041"},{"key":"e_1_2_9_3_1","first-page":"89","volume-title":"Toll\u2010Like Receptor Stimulation in Cancer: A Pro\u2010 and Anti\u2010Tumor Double\u2010Edged Sword","author":"Dajon M","year":"2017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00461"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.04.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2541"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04046-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.14639\/0392-100X-2307"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.24964"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2017.01.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2015.03.017"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3233\/TUB-211519"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-13082-8"},{"key":"e_1_2_9_14_1","article-title":"T\u2010cell intrinsic Toll\u2010like receptor signaling: implications for cancer immunotherapy and CAR T\u2010cells","volume":"9","author":"Nouri Y","year":"2021","journal-title":"JITC"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.667454"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-4971-8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.16492"},{"key":"e_1_2_9_18_1","first-page":"1","article-title":"Toll\u2010like receptor 3 in solid cancer and therapy resistance","volume":"12","author":"Muresan XM","year":"2020","journal-title":"Cancer"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513861200"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.615"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2010.00929.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.589"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12233"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21197255"},{"key":"e_1_2_9_25_1","article-title":"Trial watch: experimental TLR7\/TLR8 agonists for oncological indications","volume":"9","author":"Frega G","year":"2020","journal-title":"Onco Targets Ther"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12160"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2022.154284"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065278"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.13505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T03:54:54Z","timestamp":1753415694000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.13505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,16]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/apm.13505"],"URL":"https:\/\/doi.org\/10.1111\/apm.13505","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"type":"print","value":"0903-4641"},{"type":"electronic","value":"1600-0463"}],"subject":[],"published":{"date-parts":[[2024,12,16]]},"assertion":[{"value":"2024-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13505"}}