{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T17:48:54Z","timestamp":1755798534198,"version":"3.41.2"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF14OC0012203"],"award-info":[{"award-number":["NNF14OC0012203"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>The ovarian oncobiome is subject to increasing scientific focus, but a potential link between bacterial dysbiosis and ovarian carcinogenesis remains controversial. Our primary aim was to characterize the bacterial microbiota in epithelial ovarian cancer samples. Secondarily, we aimed to compare results from the bacterial microbiota in formalin\u2010fixed, paraffin\u2010embedded ovarian tissue samples from 194 patients with epithelial ovarian cancer, fallopian tube tissue samples from 16 patients with serous tubal intraepithelial carcinomas and in benign fallopian tube tissue samples from 25 patients. Bacterial abundance was investigated by means of 16S rDNA Next Generation Sequencing. The 16S rDNA sequencing run produced a very low number of bacterial reads. Only seven samples displayed bacterial reads above 5000. Validation of detected bacterial reads by qPCR was negative and indicative of results being background amplification. Our results indicate no amount of bacterial biomass in the ovarian cancer, benign fallopian tube and in the samples with serous tubal intraepithelial carcinomas. The findings underline the importance of validating results from bacterial microbiota studies with additional technical assays before any conclusion may be drawn.<\/jats:p>","DOI":"10.1111\/apm.13509","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T02:03:08Z","timestamp":1737079388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["No Bacterial Biomass Detected in Tissue From Patients With Ovarian Cancer and Serous Tubal Intraepithelial Carcinomas Using 16S rDNA Sequencing"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1892-2139","authenticated-orcid":false,"given":"Kasper","family":"Ingerslev","sequence":"first","affiliation":[{"name":"Department of Pathology Herlev and Gentofte University Hospital  Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim Svenstrup","family":"Poulsen","sequence":"additional","affiliation":[{"name":"Department of Pathology Herlev and Gentofte University Hospital  Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikael Lenz","family":"Strube","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wojciech","family":"Skovrider\u2010Ruminski","sequence":"additional","affiliation":[{"name":"Department of Pathology Herlev and Gentofte University Hospital  Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris","family":"Schledermann","sequence":"additional","affiliation":[{"name":"Department of Pathology Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tine Henrichsen","family":"Schnack","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Odense University Hospital  Odense Denmark"},{"name":"Department of Clinical Research University of Southern Denmark  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"H\u00f8gdall","sequence":"additional","affiliation":[{"name":"Department of Gynecology Copenhagen University Hospital  Rigshospitalet Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Blaak\u00e6r","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Odense University Hospital  Odense Denmark"},{"name":"Department of Clinical Research University of Southern Denmark  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4689-5658","authenticated-orcid":false,"given":"Estrid","family":"H\u00f8gdall","sequence":"additional","affiliation":[{"name":"Department of Pathology Herlev and Gentofte University Hospital  Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(18)32552\u20102"},{"issue":"16","key":"e_1_2_9_3_1","first-page":"4722","article-title":"Gene Expression in Ovarian Cancer Reflects Both Morphology and Biological Behavior, Distinguishing Clear Cell From Other Poor\u2010Prognosis Ovarian Carcinomas","volume":"62","author":"Schwartz D. 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