{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T19:51:07Z","timestamp":1785873067879,"version":"3.56.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Intraepithelial lymphocytes (IELs) count, central for coeliac disease (CD) diagnosis, can be performed either directly on hematoxylin and eosin (H&amp;E)\u2013stained paraffined sections or on optimal\u2010cutting\u2010temperature\u2010compound (OCT)\u2010embedded frozen sections stained by immunohistochemistry (IHC) with anti\u2010CD3. We evaluated the concordance in Marsh grading between these two techniques on a large sample of sections. A total of 280 patients with a normal intestinal architecture, 210 potential celiac disease (PCD) patients, and 70 controls (CTR) were included. At the H&amp;E histological evaluation, 136\/280 were classified as Marsh\u20100 (showing\u2009&lt;\u200925 IELs\/100 enterocytes) and 144 Marsh\u20101, while at the IHC evaluation, 191 were classified as Marsh\u20100 (showing\u2009\u2264\u200934 CD3+\/mm of epithelium) and 89 Marsh\u20101. The overall concordance was 66.8% (48.6% Marsh\u20101 and 86% Marsh\u20100) with a Cohen Kappa value of 0.33. In the PCD group, the overall concordance was 63% (45.6% Marsh\u20101 and 84% Marsh\u20100) with a Cohen Kappa value of 0.26, while in the CTR group it was 77% (60% Marsh\u20101, 90% Marsh\u20100) with a Cohen Kappa value of 0.54. Differences between the two groups were statistically significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In conclusion, the concordance of IELs counts between histological and IHC evaluation is low (Kappa Cohen 0.54) in no\u2010CD and even more in PCD patients (0.26). Caution must be paid when classifying a patient as Marsh\u20100 or Marsh\u20101 according to the technique used.<\/jats:p>","DOI":"10.1111\/apm.70015","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T07:55:53Z","timestamp":1742284553000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of Intraepithelial Lymphocytes Count in Potential Celiac Disease"],"prefix":"10.1111","volume":"133","author":[{"given":"Roberta","family":"Mandile","sequence":"first","affiliation":[{"name":"European Laboratory for the Investigation of Food Induced Diseases (ELFID) University of Naples Federico II  Naples Italy"},{"name":"Department of Translational Medical Sciences, Section of Paediatrics University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-3779","authenticated-orcid":false,"given":"Mariantonia","family":"Maglio","sequence":"additional","affiliation":[{"name":"European Laboratory for the Investigation of Food Induced Diseases (ELFID) University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Marano","sequence":"additional","affiliation":[{"name":"European Laboratory for the Investigation of Food Induced Diseases (ELFID) University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Rapacciuolo","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, Section of Paediatrics University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Discepolo","sequence":"additional","affiliation":[{"name":"European Laboratory for the Investigation of Food Induced Diseases (ELFID) University of Naples Federico II  Naples Italy"},{"name":"Department of Translational Medical Sciences, Section of Paediatrics University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Troncone","sequence":"additional","affiliation":[{"name":"European Laboratory for the Investigation of Food Induced Diseases (ELFID) University of Naples Federico II  Naples Italy"},{"name":"Department of Translational Medical Sciences, Section of Paediatrics University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Auricchio","sequence":"additional","affiliation":[{"name":"European Laboratory for the Investigation of Food Induced Diseases (ELFID) University of Naples Federico II  Naples Italy"},{"name":"Department of Translational Medical Sciences, Section of Paediatrics University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07579.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5858\/2006-130-1020-ILIAPP"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-012-0316-x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.05.013"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5409\/wjcp.v10.i4.53"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.34.2.150"},{"key":"e_1_2_10_8_1","first-page":"136","volume-title":"Coeliac Disease","author":"Marsh M. 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