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This research explores the potential of halophilic microorganisms as a source of novel antimicrobial compounds, focusing on <jats:styled-content style=\"fixed-case\"><jats:italic>Bacillus safensis<\/jats:italic><\/jats:styled-content> isolated from saltpan soils in the Tuticorin coastal region, India. Among 158 isolates, <jats:styled-content style=\"fixed-case\"><jats:italic>B. safensis<\/jats:italic><\/jats:styled-content> strain TC67 demonstrated potent anti\u2010MRSA activity and was optimized under specific growth conditions for maximal metabolite production. The active compound was purified through silica gel column chromatography and analyzed using TLC, GC\u2013MS, and <jats:sup>1<\/jats:sup>H NMR spectroscopy. These analyses identified benzene derivatives and saturated fatty acids as key components, including eicosanoic and decanoic acids. The purified metabolites exhibited a minimum inhibitory concentration (MIC) of 31.25\u2009\u03bcg\/mL against MRSA. Mechanistic studies using flow cytometry and scanning electron microscopy (SEM) confirmed that the compound disrupts MRSA cell membranes, leading to decreased cell viability. This study highlights the potential of marine\u2010derived <jats:italic>Bacillus<\/jats:italic> species as a source for antimicrobial agents, providing viable choices to combat MRSA infections.<\/jats:p>","DOI":"10.1111\/apm.70018","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T07:46:13Z","timestamp":1742283973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fatty Acids and Benzene Derivatives Partitioned From Marine\u2010Derived <scp><i>Bacillus safensis<\/i><\/scp>: Novel Agents Against Methicillin\u2010Resistant <scp><i>Staphylococcus aureus<\/i><\/scp>"],"prefix":"10.1111","volume":"133","author":[{"given":"Venkadapathi","family":"Jeyanthi","sequence":"first","affiliation":[{"name":"Department of Biotechnology SRM Arts and Science College  Chengalpattu Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeevitha","family":"Dhanasekaran","sequence":"additional","affiliation":[{"name":"Department of Biotechnology SRM Arts and Science College  Chengalpattu Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherin John","family":"Joseph","sequence":"additional","affiliation":[{"name":"Department of Biotechnology SRM Arts and Science College  Chengalpattu Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majed A.","family":"Bajaber","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science King Khalid University  Abha Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Palaniyandi","family":"Velusamy","sequence":"additional","affiliation":[{"name":"Innovation and Incubation Centre for Health Sciences, Sree Balaji Medical College and Hospital Bharath Institute of Higher Education and Research  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Periasamy","family":"Anbu","sequence":"additional","affiliation":[{"name":"Center for Global Health Research, Saveetha Medical College &amp; Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS) Thandalam  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-4687","authenticated-orcid":false,"given":"Subash C. 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