{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T10:25:52Z","timestamp":1774347952846,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Chronic rhinosinusitis with nasal polyps (CRSwNP) lacks effective therapies, highlighting miRNAs as potential therapeutic targets owing to their biological functions and analytical accessibility. This study aimed to investigate the role of miR\u2010145\u20105p in CRSwNP. Serum samples and inferior turbinate mucosa tissues were collected from 175 CRSwNP patients and 90 nasal septum deviation (NSD) subjects. qRT\u2010PCR measured miR\u2010145\u20105p expression in serum and tissue samples, and CD40 expression in tissues. ROC curve evaluated the diagnostic efficacy. Associations with disease severity and eosinophilic CRSwNP (ECRSwNP) risk were analyzed through correlation and logistic regression analysis. Dual\u2010luciferase assay confirmed miR\u2010145\u20105p targeting CD40. LPS\u2010induced inflammation in human nasal epithelial cells (hNEpCs) assessed the miR\u2010145\u20105p\/CD40 axis's impact on IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1 levels. Tissue and serum miR\u2010145\u20105p effectively diagnosed CRSwNP. Reduced miR\u2010145\u20105p correlated with disease severity and was an independent ECRSwNP risk factor. In LPS\u2010stimulated hNEpCs, miR\u2010145\u20105p overexpression suppressed IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1. CD40 expression inversely correlated with miR\u2010145\u20105p and amplified inflammatory responses mitigated by miR\u2010145\u20105p overexpression. MiR\u2010145\u20105p, serving as a diagnostic biomarker for CRSwNP, was negatively correlated with disease severity. MiR\u2010145\u20105p overexpression attenuated inflammation in hNEpCs by targeting CD40, thereby involving itself in the progression of CRSwNP, suggesting therapeutic potential for CRSwNP management.<\/jats:p>","DOI":"10.1111\/apm.70045","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T09:36:01Z","timestamp":1752831361000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>MiR<\/scp>\n                    \u2010145\u20105p Regulates Inflammatory Response via Targeting\n                    <scp>CD40<\/scp>\n                    in Chronic Rhinosinusitis With Nasal Polyposis"],"prefix":"10.1111","volume":"133","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery CR &amp; WISCO General Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangyao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery CR &amp; WISCO General Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xixia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery CR &amp; WISCO General Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery CR &amp; WISCO General Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5824-1998","authenticated-orcid":false,"given":"Xiaoyan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery CR &amp; WISCO General Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1800215"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/clt2.12002"},{"issue":"4","key":"e_1_2_8_4_1","first-page":"370","article-title":"Chronic Rhinosinusitis","volume":"108","author":"Keating M. 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