{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T10:25:52Z","timestamp":1774347952086,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["315S187"],"award-info":[{"award-number":["315S187"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n The objective was to investigate the effectiveness of anidulafungin (ANI), voriconazole (VOR), and amphotericin (AMB) combined with meropenem against dual biofilms of\n \n Candida albicans<\/jats:italic>\n <\/jats:styled-content>\n and\n \n Pseudomonas aeruginosa<\/jats:italic>\n <\/jats:styled-content>\n , as well as with daptomycin against dual biofilms of\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n \u2013\n \n Staphylococcus epidermidis<\/jats:italic>\n <\/jats:styled-content>\n . MIC values of antifungals were determined by CLSI M27\u2010A3 method. The effectiveness of antifungals in combination with antibiotics against biofilms was tested by Checkerboard analysis. Biofilm quantifications were tested by the XTT method. The biofilm images were captured using SEM. The quantity of\n C. albicans<\/jats:italic>\n \u2013\n S. epidermidis and C. albicans<\/jats:italic>\n \u2013\n P. aeruginosa<\/jats:italic>\n was lower (83.2% and 56.3%, respectively) than that of single Candida biofilm. The SMIC50 values for single\n C. albicans<\/jats:italic>\n biofilms were 32\u2010fold higher for AMB and VOR, and 128\u2010fold higher for ANI when compared to dual Candida biofilms. Daptomycin combined with ANI or VOR showed antagonistic effects against\n C. albicans<\/jats:italic>\n \u2013\n S. epidermidis<\/jats:italic>\n biofilms, while meropenem in combination with all antifungals was found to be additive against\n C. albicans<\/jats:italic>\n \u2013\n P. aeruginosa<\/jats:italic>\n biofilms. Antifungals and their combinations resulted in a decrease in blastospore and bacterial density, as observed through SEM. In\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n \u2013\n \n P. aeruginosa<\/jats:italic>\n <\/jats:styled-content>\n biofilms, using meropenem alongside antifungals would be beneficial due to its additive effect. Daptomycin combined with ANI or VOR may not be effective in\n C. albicans<\/jats:italic>\n \u2013\n S. epidermidis<\/jats:italic>\n coinfections.\n <\/jats:p>","DOI":"10.1111\/apm.70047","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T03:05:19Z","timestamp":1752635119000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Activity of Antifungals in Combination With Meropenem or Daptomycin Against Dual\n Candida albicans<\/i>\n \u2013Bacterial Biofilms"],"prefix":"10.1111","volume":"133","author":[{"given":"Agim","family":"Osmani","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Kocaeli University Faculty of Medicine Kocaeli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7238-6975","authenticated-orcid":false,"given":"Sema Ask\u0131n","family":"Keceli","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Kocaeli University Faculty of Medicine Kocaeli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doganhan Kadir","family":"Er","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Kocaeli University Faculty of Medicine Kocaeli Turkey"},{"name":"Department of Molecular Gastroenterology and Hepatology Institute of Gastroenterology and Hepatology, Kocaeli University Kocaeli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huseyin","family":"Uzuner","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques Vocational School of Health Services, Kocaeli University Kocaeli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feriha","family":"Ercan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Marmara University Faculty of Medicine Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayca Karagoz","family":"Koro\u011flu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Marmara University Faculty of Medicine Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2018.04.059"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002848"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.075"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2018.10.007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00029\u201006"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2010.01.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002121"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-11-27"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2013.08.018"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30304-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0249547"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2011.07.004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-125"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2014.55.2.449"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2006.apm_450.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-4-344"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01728-08"},{"key":"e_1_2_9_19_1","article-title":"A 96 Well Microtiter Plate\u2010Based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms","volume":"44","author":"Pierce C. 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