{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:02:11Z","timestamp":1790013731923,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    )\u2010induced osteomyelitis presents therapeutic challenges due to antibiotic resistance. Isoliquiritigenin (ISL), a licorice\u2010derived chalcone, exhibits antibacterial and anti\u2010inflammatory properties. This study evaluated vancomycin (VAN) combined with ISL against methicillin\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MRSA)\u2010induced implant\u2010related osteomyelitis. A rat model was established by tibial MRSA inoculation with simultaneous Kirschner wire implantation. Four weeks postinfection, rats were divided into five groups: normal, model, VAN (50\u2009mg\/kg), ISL (100\u2009mg\/kg), and VAN + ISL. After 14\u2009days of treatment, combined therapy significantly reduced bone infection severity and histopathological scores versus monotherapies (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), decreased serum inflammatory markers (IL\u20106, TNF\u2010\u03b1, IL\u20101\u03b2, and CRP;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), and reduced bacterial loads in bone\/wire (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). In\u00a0vitro, ISL (50\u2009\u03bcM) attenuated MRSA\u2010induced inflammatory response in MC3T3\u2010E1 osteoblasts by suppressing NF\u2010\u03baB and MAPK signaling, while promoting osteogenesis via increased Runx2\/BMP2\/ALP expression, activated BMP\/Smad signaling, and enhanced mineralization. Overall, VAN + ISL combination therapy outperforms monotherapy by concurrently eradicating MRSA, suppressing inflammation, and promoting bone repair, representing a promising synergistic strategy for recalcitrant osteomyelitis.\n                  <\/jats:p>","DOI":"10.1111\/apm.70056","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T03:56:09Z","timestamp":1758686169000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Combination of Isoliquiritigenin and Vancomycin Alleviates\n                    <scp>\n                      <i>Staphylococcus aureus<\/i>\n                    <\/scp>\n                    \u2010Induced Bone Infection in Rats"],"prefix":"10.1111","volume":"133","author":[{"given":"Mingbo","family":"Wang","sequence":"first","affiliation":[{"name":"Trauma Surgery Section B The Second Affiliated Hospital of Inner Mongolia Medical University  Hohhot 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