{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:08:49Z","timestamp":1761898129323,"version":"3.44.0"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Rapid and reliable identification of <jats:italic>Candida<\/jats:italic> spp. is crucial due to changing epidemiology and increasing resistance. This study aims to compare the identification rates, average Log Score (LS) values, and three different methods: short\u2010term culture, Sepsityper kit, and an in\u2010house method. Simulated blood culture (BC) samples with clinical <jats:italic>Candida<\/jats:italic> isolates and human blood from healthy donors were used. Sepsityper kit was used according to manufacturers' recommendations. An in\u2010house protocol was designed using SDS for lysis of erythrocytes. Short\u2010term culture was performed by inoculation of BC broth on Sabouraud Dextrose Agar (SDA) and chromogenic plates and cultured for 6\u2009h. A total of 52 clinical <jats:italic>Candida<\/jats:italic> isolates were included in the study. The identification rate was highest (71.9%) for the short\u2010term culture method, 59.6% for the Sepsityper kit, and 57.3% for the in\u2010house method when all types of bottles were analyzed. Higher identification rates were obtained using the <jats:styled-content style=\"fixed-case\">BD BACTEC<\/jats:styled-content> Mycosis\u2010<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\/F bottles: 76.7% for the short\u2010term culture method, 100% for the in\u2010house method, and 76.9% for the Sepsityper kit. Short\u2010term culture has high performance in the identification of <jats:italic>Candida<\/jats:italic> species, despite a slightly longer detection time than direct MALDI\u2010TOF MS methods.<\/jats:p>","DOI":"10.1111\/apm.70063","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T04:50:21Z","timestamp":1756183821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Performance of Short\u2010Term Culture and Direct <scp>MALDI<\/scp>\u2010<scp>TOF MS<\/scp> for Identification of <i>Candida<\/i> Species From Blood Cultures"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5318-2942","authenticated-orcid":false,"given":"Tugce","family":"Unalan\u2010Altintop","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Medical Microbiology Hacettepe University Medical School  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristoffer","family":"Jansson","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Karolinska University Hospitals  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volkan","family":"\u00d6zenci","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Clinical Microbiology Karolinska University Hospitals  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/421946"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134\u2010014\u20103310\u2010z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/378745"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010013\u20101574\u2010z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.05742\u201011"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.221770"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/827416"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw038"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114834"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01897.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000168"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284\u2010016\u20101161\u20102"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01788\u201014"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12936"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2024.116271"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2012.762607"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00339\u201011"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.03600\u201014"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.04.013"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.70063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T09:39:05Z","timestamp":1756201145000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.70063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/apm.70063"],"URL":"https:\/\/doi.org\/10.1111\/apm.70063","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"type":"print","value":"0903-4641"},{"type":"electronic","value":"1600-0463"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2024-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70063"}}