{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T19:28:12Z","timestamp":1778959692844,"version":"3.51.4"},"reference-count":56,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100031399","name":"Neotia University","doi-asserted-by":"publisher","award":["TNU\/R&D\/MG\/24\/02"],"award-info":[{"award-number":["TNU\/R&D\/MG\/24\/02"]}],"id":[{"id":"10.13039\/100031399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031399","name":"Neotia University","doi-asserted-by":"publisher","award":["TNU\/R&D\/MG\/24\/04"],"award-info":[{"award-number":["TNU\/R&D\/MG\/24\/04"]}],"id":[{"id":"10.13039\/100031399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Methicillin\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MRSA) is reported to cause serious health issues in humans by exploiting its biofilm network. To combat this global concern, the combined efficacy of cuminaldehyde (a bioactive phytochemical) and vancomycin (an antibiotic) was tested against MRSA strains. While both compounds exhibited independent antibacterial activity, their combination revealed improved efficacy against MRSA through additive interactions. Response surface methodology (RSM)\u2010generated quadratic models optimized the combinatorial doses, revealing significant microbial growth inhibition of the MRSA strains (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Furthermore, the combined application of cuminaldehyde and vancomycin at sub\u2010MIC doses could inhibit biofilm formation by lowering bacterial adhesion, extracellular polysaccharide (EP) synthesis and the extent of biofilm\u2010associated proteins. Additionally, the mechanistic studies revealed that the said combination (cuminaldehyde and vancomycin) was found to accumulate oxidative stress with a ~2.5\u2010fold increase in intracellular reactive oxygen species (ROS) and a ~2.3\u2010fold reduction in membrane integrity. In view of the same, this combination attenuated key virulence factors (protease, hemolysin, and coagulase) and metabolic activity of MRSA. Hence, the combinations involving cuminaldehyde and vancomycin could potentially enhance the antimicrobial and antibiofilm efficacy, presenting a promising approach to combat the escalating crisis linked with MRSA\u2010associated threats.\n                  <\/jats:p>","DOI":"10.1111\/apm.70087","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:37:31Z","timestamp":1763728651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cuminaldehyde Potentiates the Antimicrobial and Antibiofilm Activity of Vancomycin: A Biochemical Study to Manage the Threats of Methicillin Resistant\n                    <i>Staphylococcus aureus<\/i>\n                    (\n                    <scp>MRSA<\/scp>\n                    )"],"prefix":"10.1111","volume":"133","author":[{"given":"Saranya","family":"Trivedi","sequence":"first","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritwik","family":"Roy","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Animesh","family":"Naskar","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology Maulana Abul Kalam Azad University of Technology  Haringhata West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biswamitra","family":"Das","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poulomi","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Payel","family":"Paul","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharmistha","family":"Das","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moumita","family":"Malik","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6567-006X","authenticated-orcid":false,"given":"Prosun","family":"Tribedi","sequence":"additional","affiliation":[{"name":"Microbial Ecology Research Laboratory, Department of Biotechnology The Neotia University  Sarisha West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4065\/mcp.2011.0514"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00134-14"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-024-03380-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-015-1148-6"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9MD00044E"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2016.08.007"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2011.09.028"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00007709"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.09.123"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1397040"},{"key":"e_1_2_8_12_1","first-page":"1223","article-title":"The Study of Antimicrobial Sensitivity Patterns of VRSA, Visa and VSSA Isolates Obtained From Various Clinical Specimens Among MRSA","volume":"14","author":"Ahmed R.","year":"2024","journal-title":"European Journal of Cardiovascular Medicine"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/hygiene4030024"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.106650"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxaf211"},{"issue":"2","key":"e_1_2_8_16_1","first-page":"10","article-title":"Evaluating the Efficacy of T. 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