{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:04:56Z","timestamp":1788282296118,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["ICAR\/Edn.10(8)\/2016\u2010EP&HS"],"award-info":[{"award-number":["ICAR\/Edn.10(8)\/2016\u2010EP&HS"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antimicrobial\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , including methicillin\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MRSA), is an increasing concern in dairy production. Using a One Health approach, this cross\u2010sectional study assessed the prevalence, antimicrobial resistance (AMR) profiles, and biofilm\u2010forming ability of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    from pooled bovine milk, animal handler hand swabs, and herd slurry collected from 405 farms across Punjab, India. The prevalence of coagulase\u2010positive\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    was highest in hand swabs (43.7%), followed by milk (30.1%) and slurry (17.5%). Phenotypic resistance varied by source: milk isolates showed the greatest resistance to cefoxitin (37.7%) and penicillin (36.9%); hand swab isolates showed resistance to erythromycin (42.9%) and tetracycline (36.7%); and slurry isolates showed resistance to tetracycline (66.2%) and cefoxitin (59.2%). Multidrug resistance (MDR) was found in 45.9% of milk, 60.5% of hand swabs, and 92.9% of slurry isolates. MRSA was detected in 6.6% of milk, 2.8% of hand swab, and 1.4% of slurry isolates, with SCCmec type V as the most common type. Genotypic screening identified\n                    <jats:italic>blaZ<\/jats:italic>\n                    ,\n                    <jats:italic>mecA<\/jats:italic>\n                    ,\n                    <jats:italic>tetK<\/jats:italic>\n                    ,\n                    <jats:italic>ermC<\/jats:italic>\n                    , and\n                    <jats:italic>aacA\u2010aphD<\/jats:italic>\n                    , with the highest genotype\u2013phenotype concordance for tetracycline resistance. Biofilm assays showed 94.9% of isolates formed biofilms; 28.4% were strong producers. MDR milk isolates showed the highest strong biofilm capacity (39.3%). This study underscores the need for integrated AMR surveillance and improved dairy biosecurity.\n                  <\/jats:p>","DOI":"10.1111\/apm.70089","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T10:48:01Z","timestamp":1762944481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mapping Prevalence, Antimicrobial Resistance, and Biofilm Production of Dairy Farm Associated Coagulase\u2010Positive\n                    <scp>\n                      <i>Staphylococcus aureus<\/i>\n                    <\/scp>\n                    From Punjab, India"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7384-8123","authenticated-orcid":false,"given":"Deepthi","family":"Vijay","sequence":"first","affiliation":[{"name":"Centre for One Health, College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana 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