{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:09:32Z","timestamp":1772989772636,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To investigate, through immunohistochemistry, the interaction between the epithelial\u2010mesenchymal transition (EMT) status and the tumor microenvironment (TME) in oral tongue squamous cell carcinomas (OTSCCs). A total of 61 cases of OTSCC were selected. In all cases, the EMT status was determined through the analysis of E\u2010cadherin and N\u2010cadherin immunoexpression, while the TME was characterized using TGF\u2010\u03b2 and \u03b1\u2010SMA for cancer\u2010associated fibroblasts, CD163 for M2 macrophages, and Foxp3 for regulatory T cells. Associations between the expression patterns of these proteins and clinicopathological features, as well as overall survival (OS) and disease\u2010free survival (DFS) parameters, were investigated. High \u03b1\u2010SMA expression in OTSCC cases was significantly associated with a positive EMT status (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.011). This marker also showed a significant positive correlation with TGF\u2010\u03b2 immunostaining (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.028). Foxp3 and CD163 also demonstrated a significant positive correlation (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.010). Multivariate analysis identified high Foxp3 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.011) and TGF\u2010\u03b2 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.002) expression as independent prognostic factors for OS, and high TGF\u2010\u03b2 expression (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.031) as an independent prognostic factor for DFS. The results suggest an interaction among TME biomarkers, with implications for EMT development and progression, as well as for the prognosis of OTSCC.\n                  <\/jats:p>","DOI":"10.1111\/apm.70126","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T15:00:47Z","timestamp":1765983647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Interaction Between Epithelial\u2010Mesenchymal Transition and Tumor Microenvironment Biomarkers and Their Impact on the Prognosis of Oral Tongue Squamous Cell Carcinoma"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3488-3465","authenticated-orcid":false,"given":"Hannah Gil de Farias","family":"Morais","sequence":"first","affiliation":[{"name":"Department of Oral Pathology Federal University of Rio Grande do Norte  Natal Brazil"},{"name":"Federal University of Rio Grande do Norte Multicampi School 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Anatomopathological Diagnosis (CEDAPP) Affiliated With Napole\u00e3o Laureano Hospital and the Pathology Laboratory of Lauro Wanderley University Hospital  Jo\u00e3o Pessoa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Everton Freitas","family":"de Morais","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry of Piracicaba State University of Campinas  Piracicaba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4449-4343","authenticated-orcid":false,"given":"Paulo Rogerio Ferreti","family":"Bonan","sequence":"additional","affiliation":[{"name":"Graduate Program in Dentistry Federal University of Para\u00edba  Jo\u00e3o Pessoa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roseana de Almeida","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Federal University of Rio Grande do Norte  Natal 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