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This study optimized LBC block preparation by evaluating five methods using 90 cervical samples. Metrics included visual assessment, hematoxylin and eosin (H&amp;E) staining, Immunohistochemistry (IHC) performance, and production efficiency. The modified adhesive centrifugal sedimentation method demonstrated superior outcomes, yielding well\u2010preserved cell clusters with enhanced H&amp;E contrast (reduced impurities) and precise IHC localization. Its preparation success rate reached 92.9%. Validation with 114 cervical cases showed cell blocks achieved 87.5% sensitivity and 96.9% specificity compared to biopsy histology, outperforming standard LBC. The method's simplicity, cost\u2010effectiveness, and cell preservation advantages support its utility in primary settings for cervical lesion screening. Findings highlight LBC blocks as a reliable adjunct to cytology, improving diagnostic precision while maintaining workflow feasibility.<\/jats:p>","DOI":"10.1111\/apm.70131","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T03:41:16Z","timestamp":1767757276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of Liquid\u2010Based Cytology Block Preparation Using Modified Adhesive Centrifugal Sedimentation for Improved Diagnostic Accuracy in Cervical Lesions"],"prefix":"10.1111","volume":"134","author":[{"given":"Ran","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingli","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manli","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xunjian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8898-1426","authenticated-orcid":false,"given":"Hua","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiyan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology, the Affiliated Changsha Hospital of Xiangya School of Medicine Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,6]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"S2","article-title":"The Impact of Liquid\u2010Based Cytology in Decreasing the Incidence of Cervical Cancer","volume":"4","author":"Gibb R. 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