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However, the inappropriate or excessive use of antibiotics has significantly contributed to the emergence and spread of resistant strains, posing a growing public health challenge. In this context, this study aimed to taxonomically identify and assess the antibacterial potential of the CS05 and CS83 bacterial isolates, obtained from the human conjunctival sac. The latter was accomplished through assays designed to evaluate their ability to inhibit the growth of reference pathogenic strains from the American Type Culture Collection. Antibacterial activity assays, including cross\u2010diffusion, drop agar diffusion, well diffusion, disk diffusion, and polystyrene microplate assays, were performed, and taxonomic identification was carried out by Sanger sequencing. The bacteria CS05 was identified as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    <\/jats:styled-content>\n                    and demonstrated inhibitory activity against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus cereus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Meanwhile, the isolate CS83, identified as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n                    <\/jats:styled-content>\n                    , inhibited the growth of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . These findings suggest that the bacterial microbiota of the human conjunctival sac may represent a promising biotechnological source to develop alternative antibacterial agents.\n                  <\/jats:p>","DOI":"10.1111\/apm.70133","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T03:32:23Z","timestamp":1767756743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibacterial Activity of\n                    <i>Staphylococcus epidermidis<\/i>\n                    <scp>CS05<\/scp>\n                    and\n                    <i>Pseudomonas fluorescens<\/i>\n                    <scp>CS83<\/scp>\n                    Isolated From the Human Conjunctival Sac Against Pathogenic Bacterial Reference Strains"],"prefix":"10.1111","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-7069","authenticated-orcid":false,"given":"Joicye","family":"Hern\u00e1ndez\u2010Zulueta","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Instituto de Fisiolog\u00eda Celular, Departamento de Biolog\u00eda Celular y Molecular Centro Universitario de Ciencias Biol\u00f3gicas y Agropecuarias, Universidad de Guadalajara  Zapopan Jalisco M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5283-6000","authenticated-orcid":false,"given":"Alejandro","family":"Gonz\u00e1lez\u2010De la Rosa","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico de Monterrey Escuela de Medicina y Ciencias de la Salud  Zapopan Jalisco M\u00e9xico"},{"name":"Centro de Retina Medica y Quir\u00fargica, S.C. 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