{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:52:01Z","timestamp":1773017521303,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study identified miR\u20105701 and TP53 through bioinformatics analysis utilizing the GEO, GeneCards, and miRDB databases. The serum expression levels were quantified via Reverse Transcription Quantitative Real\u2010time Polymerase Chain Reaction (RT\u2010qPCR). The binding relationship between miR\u20105701 and TP53 was confirmed using dual luciferase reporter assays. Subsequently, MC3T3\u2010E1 cell differentiation was induced with Bone Morphogenetic Protein 2 (BMP2), and the expression changes of miR\u20105701 and TP53 after cell differentiation were detected. Subsequently, after overexpression of miR\u20105701 and TP53, the expression levels of key osteogenic differentiation markers Alkaline Phosphatase (ALP), Osteocalcin (OCN), and type I collagen were evaluated by ELISA. Serum miR\u20105701 expression is downregulated in osteoporotic fracture patients, while it increases after osteoblast differentiation. TP53 is a negative regulatory binding factor downstream of miR\u20105701. The mRNA expression of TP53 in the patient serum and the differentiated osteoblast cells is exactly opposite to that of miR\u20105701. Overexpression of miR\u20105701\u2010mediated promotion of osteoblast proliferation and differentiation can be reversed by TP53. miR\u20105701 was found to facilitate osteoblast proliferation and differentiation through negative regulation of the target gene TP53 expression, suggesting that miR\u20105701 could serve as a promising therapeutic target for promoting fracture healing.<\/jats:p>","DOI":"10.1111\/apm.70153","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T10:12:09Z","timestamp":1770027129000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR\u20105701 Drives Bone Fracture Repair in Osteoporosis Through TP53 Regulation"],"prefix":"10.1111","volume":"134","author":[{"given":"Sinan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Health Medicine University of Sanya  Sanya China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Huanggang Central Hospital  Huanggang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5198-1316","authenticated-orcid":false,"given":"Ganggang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hand and Foot Surgery Zhucheng People's Hospital  Weifang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1730-3691","authenticated-orcid":false,"given":"Ningning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Affiliated Hospital of Hebei University of Engineering  Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7103-474X","authenticated-orcid":false,"given":"Huiling","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation The Affiliated Hospital of Youjiang Medical University for Nationalities  Baise China"},{"name":"Guangxi Key Laboratory for Preclinical and Translational Research on Bone and Joint Degenerative Diseases  Baise Guangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI68833"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.1900633"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650090802"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11657-022-01073-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2301"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-007-0343-y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000000451"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-023-04051-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005131-200501000-00004"},{"issue":"6","key":"e_1_2_9_11_1","first-page":"8269","article-title":"Effects of Nicotine on the Metabolism and Gene Expression Profile of Sprague\u2010Dawley Rat Primary Osteoblasts","volume":"17","author":"Liang D.","year":"2018","journal-title":"Molecular Medicine Reports"},{"issue":"3","key":"e_1_2_9_12_1","first-page":"1029","article-title":"Perspectives on Osteoarthritis Treatment With Mesenchymal Stem Cells and Radix Achyranthis Bidentatae","volume":"15","author":"An X.","year":"2024","journal-title":"Aging and Disease"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.897044"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3708"},{"issue":"2","key":"e_1_2_9_15_1","first-page":"456","article-title":"MicroRNA\u20109\u20105p Promotes Osteoporosis Development Through Inhibiting Osteogenesis and Promoting Adipogenesis via Targeting Wnt3a","volume":"23","author":"Zhang H. 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