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Antifungal agents may become ineffective in the future due to the rapid emergence of antifungal resistance, potentially rendering them unable to treat biofilm\u2010associated infections. The present investigation employs various in\u00a0vitro techniques, such as antifungal susceptibility, in\u00a0vitro pharmacodynamics, advanced electron microscopy, zeta potential assessment, and rhodamine 6G efflux assay, to evaluate the anti\u2010\n                    <jats:italic>Candida<\/jats:italic>\n                    and anti\u2010biofilm activities of the\n                    <jats:italic>Bacillus<\/jats:italic>\n                    \u2010derived lipopeptide against planktonic cells and mature fungal biofilms. Time\u2010kill assays indicated a significant reduction in CFUs, while scanning and transmission electron microscopy revealed pronounced ultrastructural damage and surface deformation in lipopeptide\u2010treated cells. A significant reduction in mature\n                    <jats:italic>Candida<\/jats:italic>\n                    biofilms determined by resazurin and safranin\u2010based assays was observed at different concentrations, comparable to amphotericin B. In both lipopeptide\u2010 and amphotericin B\u2010treated mature biofilms, the entangled network of massive hyphae was absent compared to the controls. COMSTAT analysis indicated a marked reduction in lipopeptide\u2010treated biofilm biomass, and confocal microscopy results corroborated these observations.\n                  <\/jats:p>","DOI":"10.1111\/apm.70163","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T11:11:49Z","timestamp":1771499509000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Understanding the Antifungal and Antibiofilm Potential of a Naturally Occurring\n                    <i>Bacillus<\/i>\n                    Lipopeptide"],"prefix":"10.1111","volume":"134","author":[{"given":"Madhuri","family":"Madduri","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Birla Institute of Technology &amp; Scienc, Pilani, K.K. 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