{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:09:36Z","timestamp":1773029376671,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antimicrobial resistance in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    (KP) has become a major global public health threat. Allicin, a natural antimicrobial agent, has demonstrated antibacterial activity against KP, but its effect on drug resistance remains unclear. To investigate whether allicin can weaken KP's resistance to meropenem (MEM) and reveal its synergistic mechanism, this study treated KP strains with gradient concentrations of MEM. Resistant strains were identified using the broth microdilution method and viable bacterial counting. Checkerboard assay, time\u2010kill kinetics and isobologram analyses were used to analyse the antibacterial effect of different concentrations of allicin and MEM combined treatment. Molecular docking and cellular thermal shift assay (CETSA) were employed to evaluate the binding of allicin to carbapenemase and efflux pump\u2010related proteins. A mouse model of KP pneumonia was established to investigate the therapeutic effect of allicin combined with MEM. Compared with MEM monotherapy, allicin\u2009+\u2009MEM acted synergistically, lowering the effective MEM dose by targeting carbapenemase and efflux pumps and mitigating KP\u2010induced pneumonia. This study suggests that the combination of allicin and MEM may be a potential method for reducing bacterial resistance and exerting antibacterial efficacy.\n                  <\/jats:p>","DOI":"10.1111\/apm.70164","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T06:42:11Z","timestamp":1771396931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Allicin Targets Carbapenemase and Efflux Pump Activity in\n                    <i>Klebsiella pneumoniae<\/i>\n                    to Mitigate Meropenem Resistance"],"prefix":"10.1111","volume":"134","author":[{"given":"Luxiang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Hepatology and Infectious Diseases Lishui Hospital of Wenzhou Medical University, the First Affiliated Hospital of Lishui University, Lishui People's Hospital  Lishui 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