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The characteristic morphological transition and complex biofilm formation significantly contribute to its virulence and resistance to antifungal agents. This increases the pressing demand for new, safe antifungal agents. This study examined the anti\u2010biofilm and anti\u2010virulence activities of C\u2010Phycocyanin (C\u2010PC), a natural photosynthetic pigment protein produced from Spirulina. C\u2010PC demonstrated an inhibitory effect on biofilm formation in a dose\u2010dependent manner with a minimal biofilm inhibitory concentration (MBIC) of 256\u2009\u03bcg\/mL with less fungicidal action against planktonic\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    cells. The yeast\u2010to\u2010hyphae transition is crucial to its virulence and is a critically important virulence trait for pathogenic morphogenesis. This transition in morphogenesis has been disturbed by C\u2010PC treatment, suggesting that C\u2010PC challenges its ability to invade and survive. It also demonstrated decreased biofilm\u2010associated characteristics, including wrinkle development, filamentous growth, and exopolysaccharide production. Furthermore, C\u2010PC interfered with the defense system of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    by significantly reducing agar invasion, decreasing secreted phospholipase activity, and promoting oxidative stress by reducing catalase production. The obtained results collectively show that C\u2010PC could be a potential natural compound that alters key virulence traits in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/apm.70170","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:01:23Z","timestamp":1773795683000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Antibiofilm Efficacy of C\u2010Phycocyanin From Spirulina Species Against\n                    <i>Candida albicans<\/i>"],"prefix":"10.1111","volume":"134","author":[{"given":"Malik Basha","family":"Samsudeen","sequence":"first","affiliation":[{"name":"Department of Biotechnology Alagappa University  Karaikudi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hari Prasath","family":"Nagaiah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Alagappa University  Karaikudi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aishwarya","family":"Sundararaj","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Alagappa University  Karaikudi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2925-0575","authenticated-orcid":false,"given":"Karutha Pandian","family":"Shunmugiah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Alagappa University  Karaikudi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00756"},{"key":"e_1_2_9_3_1","unstructured":"Centers for Disease 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