{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T02:22:38Z","timestamp":1774491758257,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pediatric\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    pneumonia (MPP) is often complicated by digestive system damage (DSD), with unclear mechanisms and biomarkers. The role of miR\u20101224\u20105p in this remains unknown. To explore the diagnostic value, mechanism, and role of miR\u20101224\u20105p in lung\u2010gut axis injury in MPP\u2009+\u2009DSD. Bioinformatics analysis screened miR\u20101224\u20105p. Serum levels were detected via RT\u2010qPCR in 120 MPP children (57 with DSD) and 81 controls. ROC curves and logistic regression assessed its diagnostic value. LAMPs\u2010induced cell models and co\u2010cultures were used to explore the mechanisms. GO\/KEGG\u2010analyzed pathways. Dual\u2010luciferase assay was used to verify the binding relationship of the target. Serum miR\u20101224\u20105p was significantly upregulated in MPP children, especially in those complicated by DSD. MiR\u20101224\u20105p showed good diagnostic efficacy for MPP and MPP\u2009+\u2009DSD, and was an independent risk factor for DSD. In LAMPs\u2010induced BEAS\u20102B cells and co\u2010culture systems, miR\u20101224\u20105p expression was upregulated, while its inhibition reduced apoptosis and the secretion of inflammatory factors (IL\u20106, IL\u20101\u03b2, TNF\u2010\u03b1). Its predicted target genes are involved in endothelial barrier, and GRB2 is a target of miR\u20101224\u20105p. MiR\u20101224\u20105p is a potential biomarker for MPP\u2009+\u2009DSD, and it participates in lung\u2010gut injury by regulating inflammation\/barrier function.\n                  <\/jats:p>","DOI":"10.1111\/apm.70171","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:44:41Z","timestamp":1774485881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnosis and Mechanism of\n                    <scp>miR<\/scp>\n                    \u20101224\u20105p in Children With\n                    <scp>\n                      <i>Mycoplasma pneumoniae<\/i>\n                    <\/scp>\n                    Pneumonia Complicated by Digestive System Damage"],"prefix":"10.1111","volume":"134","author":[{"given":"Xin","family":"He","sequence":"first","affiliation":[{"name":"Department of Pediatric Xinchang County People's Hospital  Shaoxing City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The People's Hospital of Lincang  Lincang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1431-100X","authenticated-orcid":false,"given":"Xuening","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Xi'an Children's Hospital  Xi'an City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The Affiliated Hospital of Youjiang Medical University for Nationalities  Baise China"},{"name":"Key Laboratory of Research and Development on Clinical Molecular Diagnosis for High\u2010Incidence Diseases of Baise  Baise China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"394","article-title":"Mycoplasma pneumoniae Infections in Hospitalized Children, United States, 2018\u20132024","volume":"74","author":"Maureen H. 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