{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T04:05:45Z","timestamp":1779768345213,"version":"3.53.1"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T00:00:00Z","timestamp":1778976000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Genetic manipulation is widely employed in laboratories for purposes such as gene function characterization, the development of novel traits in transformed microorganisms, and the production of biotechnologically relevant compounds. A key step in this process is the selection of mutant strains, often facilitated by antibiotics. Tetracyclines are commonly used; however, their aqueous solutions tend to precipitate and turn brown over time, potentially compromising stability and efficacy. This necessitates frequent in\u00a0situ preparation. The present study aimed to evaluate the functional stability of tetracycline hydrochloride for selecting transformed tetracycline\u2010resistant bacterial strains in both liquid and solid media. A tetracycline stock solution and supplemented agar plates were prepared and used to cultivate resistant and non\u2010resistant strains of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Arcobacter butzleri<\/jats:italic>\n                    <\/jats:styled-content>\n                    . This allowed for assessment of antibiotic stability and utility over a 200\u2010day period. Additionally, the minimal inhibitory concentration (MIC) of tetracycline was determined for each strain. Results indicated that the tetracycline\u2010containing materials maintained functional stability for at least 175\u2009days post\u2010preparation, effectively supporting the selection of resistant strains in genetic manipulation experiments. These findings obviate the need for the continual and time\u2010consuming preparation of tetracycline\u2010supplemented culture media, thereby enhancing the practicality of employing this antibiotic in genetic modification studies.\n                  <\/jats:p>","DOI":"10.1111\/apm.70222","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T05:42:33Z","timestamp":1779082953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Stability of Tetracycline: Practical Information for Its Use in Bacterial Selection"],"prefix":"10.1111","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2454-4753","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Salazar\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy University of the Basque Country UPV\/EHU  Vitoria\u2010Gasteiz Spain"},{"name":"Bioaraba, Microbiology, Infectious Disease, Antimicrobial Agents, and Gene Therapy  Vitoria\u2010Gasteiz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8867-1487","authenticated-orcid":false,"given":"Ilargi","family":"Mart\u00ednez\u2010Ballesteros","sequence":"additional","affiliation":[{"name":"MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy University of the Basque Country UPV\/EHU  Vitoria\u2010Gasteiz Spain"},{"name":"Bioaraba, Microbiology, Infectious Disease, Antimicrobial Agents, and Gene Therapy  Vitoria\u2010Gasteiz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6576-6707","authenticated-orcid":false,"given":"Irati","family":"Martinez\u2010Malaxetxebarria","sequence":"additional","affiliation":[{"name":"MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy University of the Basque Country UPV\/EHU  Vitoria\u2010Gasteiz Spain"},{"name":"Bioaraba, Microbiology, Infectious Disease, Antimicrobial Agents, and Gene Therapy  Vitoria\u2010Gasteiz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10122454"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2022.136751"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511800986"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1201\/b18484"},{"key":"e_1_2_11_6_1","first-page":"1250","volume-title":"Basic & Clinical Pharmacology","author":"Katzung B. G.","year":"2018"},{"key":"e_1_2_11_7_1","first-page":"1115","volume-title":"Bailey & Scott's Diagnostic Microbiology","author":"Tille P. M.","year":"2017"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01058"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a025387"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.4.387"},{"key":"e_1_2_11_11_1","unstructured":"M. C.Roberts \u201cMechanism of Resistance for Characterized Tet and Otr Gene \u201d (2021) https:\/\/faculty.washington.edu\/marilynr\/tetweb1.pdf."},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.54846\/jshap\/654"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2004.10.015"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008909708650"},{"key":"e_1_2_11_15_1","unstructured":"EUCAST \u201cBreakpoint Tables for Interpretation of MICs and Zone Diameters. Version 14.0 \u201d European Committee on Antimicrobial Susceptibility Testing (2024) https:\/\/www.eucast.org\/ast_of_bacteria\/previous_versions_of_documents."},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06595-0"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01003-22"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/03601230903404556"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0442.2000.00300.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20836"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22695"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.70222","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apm.70222","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.70222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T03:52:51Z","timestamp":1779767571000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.70222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/apm.70222"],"URL":"https:\/\/doi.org\/10.1111\/apm.70222","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70222"}}