{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T06:01:11Z","timestamp":1785218471946,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["AZV NU22J\u201006\u201000058"],"award-info":[{"award-number":["AZV NU22J\u201006\u201000058"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Serum collagen IV (Col IV) is an important marker for staging liver fibrosis, but histological baselines in healthy tissue are often lacking or biased. This study applied unbiased stereological sampling to quantify liver Col IV in healthy adult Wistar rats and assess sex differences. No significant differences were observed between sampling strategies (simple, systematic, or stratified random) or between males (4.26%\u2009\u00b1\u20090.93%) and females (3.71%\u2009\u00b1\u20091.27%), yielding a pooled mean of 3.96%\u2009\u00b1\u20091.11%. The Col IV\u2010positive area (3.62%\u2009\u00b1\u20091.29%) did not differ significantly from the total collagen area measured by Sirius red (3.06%\u2009\u00b1\u20091.72%), although a statistical trend was noted (R\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.5845,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0767). These data indicate that IHC\u2010based area measurements capture a larger structural footprint of the mesh\u2010like Col IV network than biochemical mass\u2010based methods, and that 58% of the variance in Col IV area is explained by Sirius red\u2010positive area, suggesting a regulated spatial balance. The absence of sex effects on Col IV implies that previously reported sex\u2010related differences in total liver collagen likely involve other collagen types. Unbiased Col IV quantification may support validation of minimally invasive methods for staging liver fibrosis.\n                  <\/jats:p>","DOI":"10.1111\/apm.70236","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T08:56:44Z","timestamp":1783933004000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["No Sex Differences in the Amount of Type\n                    <scp>IV<\/scp>\n                    Collagen in Wistar Rats Regardless of Sampling Strategy"],"prefix":"10.1111","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9780-650X","authenticated-orcid":false,"given":"Patrik","family":"Mik","sequence":"first","affiliation":[{"name":"Department of Anatomy Faculty of Medicine in Pilsen, Charles University  Pilsen Czech Republic"},{"name":"Department of Histology and Embryology Faculty of Medicine in Pilsen, Charles University  Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsiaryna","family":"Barannikava","sequence":"additional","affiliation":[{"name":"Department of Anatomy Faculty of Medicine in Pilsen, Charles University  Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Surkova","sequence":"additional","affiliation":[{"name":"Department of Anatomy Faculty of Medicine in Pilsen, Charles University  Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3546-808X","authenticated-orcid":false,"given":"Richard","family":"P\u00e1lek","sequence":"additional","affiliation":[{"name":"Biomedical Center, Faculty of Medicine in Pilsen, Charles University  Pilsen Czech Republic"},{"name":"Department of Surgery University Hospital in Pilsen  Pilsen Czech 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