{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:59:42Z","timestamp":1790319582867,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008257","name":"Tarbiat Modares University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008257","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multiple myeloma (MM) is a hematologic malignancy characterized by uncontrolled malignant plasma cell proliferation. B\u2010cell maturation antigen (BCMA) is an attractive therapeutic target due to its high expression on malignant plasma cells. In this study, BCMA\u2010directed CAR\u2010NK\u201092 cells were generated via lentiviral transduction of a second\u2010generation CAR construct. CAR expression was confirmed by flow cytometry (81% efficiency). Engineered cells were functionally evaluated against BCMA\u2010positive (U266, RPMI\u20108226) myeloma cell lines, as well as BCMA\u2010negative control cells (K562, Jurkat). CAR\u2010NK\u201092 cells demonstrated significantly enhanced cytotoxic activity against BCMA\u2010positive targets compared with parental NK\u201092 cells (e.g., 87% vs. 54% cytotoxicity at 1:1,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Enhanced antitumor activity was accompanied by increased CD107\u03b1 surface expression and elevated secretion of perforin, granzyme B, TNF\u2010\u03b1, and IFN\u2010\u03b3 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). No significant differences in cytotoxicity or cytokine secretion were observed against BCMA\u2010negative control cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), supporting antigen\u2010specific activity. Importantly, CAR\u2010NK\u201092 cells also exhibited potent cytotoxicity and significantly elevated cytokine secretion against primary CD138+ myeloma cells isolated from patient bone marrow aspirates. These findings demonstrate that anti\u2010BCMA CAR\u2010NK\u201092 cells exhibit potent and selective antimyeloma activity in\u00a0vitro, supporting CAR\u2010NK\u201092 cells as an off\u2010the\u2010shelf immunotherapeutic platform for multiple myeloma.\n                  <\/jats:p>","DOI":"10.1111\/apm.70256","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:16:52Z","timestamp":1788337012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti\u2010\n                    <scp>BCMA CAR<\/scp>\n                    \u2010\n                    <scp>NK<\/scp>\n                    Cells Reveal Enhanced Cytolytic and Secretory Responses Against Myeloma Cells"],"prefix":"10.1111","volume":"134","author":[{"given":"Bita","family":"Divsalar","sequence":"first","affiliation":[{"name":"Department of Hematology, Faculty of Medical Sciences Tarbiat Modares University (TMU)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaffar","family":"Kiani","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeid","family":"Abroun","sequence":"additional","affiliation":[{"name":"Department of Hematology, Faculty of Medical Sciences Tarbiat Modares University (TMU)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1621-821X","authenticated-orcid":false,"given":"Masoud","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Hematology, Faculty of Medical Sciences Tarbiat Modares University (TMU)  Tehran 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