{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:04:48Z","timestamp":1790672688698,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug\u2010resistant (MDR)\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n                    <\/jats:styled-content>\n                    threatens healthcare and agriculture. Toxicity and environmental risks limit conventional plasmid\u2010curing agents, prompting natural alternatives. Curcumin, piperine, and quercetin were tested for plasmid\u2010curing against clinically isolated MDR\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. tumefaciens<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Morphological and 16S rRNA gene sequencing were used for identification of the isolate. Antimicrobial susceptibility and biofilm formation were measured pre\u2010 and post\u2010treatment. Molecular docking was performed to analyze phytocompound\u2013plasmid maintenance protein interactions. Broad\u2010spectrum resistance and ethidium bromide insensitivity were found in the MDR isolate. Curcumin, piperine, and quercetin induced plasmid curing by restoring antibiotic susceptibility and inhibiting biofilm formation. The observed increases in antibiotic susceptibility following treatment were statistically significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Molecular docking showed strong binding affinities for quercetin and tetracycline, supporting these effects' mechanism. These results show that natural compounds can eliminate MDR\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. tumefaciens<\/jats:italic>\n                    <\/jats:styled-content>\n                    biofilm formation and plasmid\u2010mediated resistance. Curcumin, piperine, and quercetin disrupt plasmid stability through protein\u2013ligand interactions, offering a promising alternative to traditional curing agents. Curcumin, piperine, and quercetin are eco\u2010friendly, effective plasmid\u2010curing agents that may combat\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. tumefaciens<\/jats:italic>\n                    <\/jats:styled-content>\n                    multidrug resistance. This integrated in\u00a0vitro and in silico approach supports phytocompound research and antibiotic\u2010resistant pathogen treatment.\n                  <\/jats:p>","DOI":"10.1111\/apm.70260","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:27:51Z","timestamp":1790670471000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated In\u00a0Vitro and In Silico Evaluation of Natural Plasmid\u2010Curing Agents Against Multidrug\u2010Resistant\n                    <i>Agrobacterium tumefaciens<\/i>"],"prefix":"10.1111","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5694-6370","authenticated-orcid":false,"given":"Shahid","family":"Attar","sequence":"first","affiliation":[{"name":"Department of Microbiology ADT's Shardabai Pawar Mahila Arts, Commerce and Science College  Shardangar, Baramati, Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3243-3763","authenticated-orcid":false,"given":"Yogesh","family":"Phatake","sequence":"additional","affiliation":[{"name":"Department of Microbiology ADT's Shardabai Pawar Mahila Arts, Commerce and Science College  Shardangar, Baramati, Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6409-3013","authenticated-orcid":false,"given":"Rajendra","family":"Marathe","sequence":"additional","affiliation":[{"name":"Department of Microbiology ADT's Shardabai Pawar Mahila Arts, Commerce and Science College  Shardangar, Baramati, Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"R.Minocha \u201cVarious Pathogens Increasingly Becoming Resistant to Critically Important Antimicrobials: ICMR Report \u201dDown to Earth (2024) accessed June 10 2024 https:\/\/www.downtoearth.org.in\/news\/health\/various\u2010pathogens\u2010increasingly\u2010becoming\u2010resistant\u2010to\u2010critically\u2010important\u2010antimicrobials\u2010icmr\u2010report\u20102024."},{"key":"e_1_2_12_3_1","unstructured":"ETPharma \u201cSustained Rise in Antimicrobial Resistance in India: ICMR Study \u201d(2024) accessed June 10 2024 https:\/\/www.etpharma.com\/news\/antimicrobial\u2010resistance\u2010icmr\u2010study\u20102024."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.51.1.223"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.12.008"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.999988"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756\u2010022\u201001086\u2010y"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00735"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201033667\u20103"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121313"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11030459"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6855"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40816\u2010021\u201000292\u20102"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27082498"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16071020"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010142"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.70041"},{"key":"e_1_2_12_18_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/9781555817381","volume-title":"Manual of Clinical Microbiology","author":"Jorgensen J. 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