{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T12:51:04Z","timestamp":1791636664364,"version":"4.3.4"},"reference-count":11,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:00:00Z","timestamp":1791590400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Alterations in the gut microbiota have been implicated in autism spectrum disorder (ASD), but reported microbial profiles remain inconsistent, potentially because of heterogeneity in factors affecting gut microbial composition. Because\n \n Helicobacter pylori<\/jats:italic>\n <\/jats:styled-content>\n infection is associated with alterations in the gut microbiota, we investigated whether ASD was associated with gut microbial composition in a cohort of\n \n H. pylori<\/jats:italic>\n <\/jats:styled-content>\n \u2010positive adolescents. This cross\u2010sectional study included seven adolescents with ASD and 80 controls before\n \n H. pylori<\/jats:italic>\n <\/jats:styled-content>\n eradication therapy. Gut microbiota was analyzed using 16S rRNA gene sequencing, with alpha diversity, centered log\u2010ratio\u2010transformed genus\u2010level abundance, and overall community composition based on Aitchison distance evaluated between groups. No significant differences were observed in alpha diversity or overall microbial community composition. At the genus level, 82 genera met the prevalence criterion for individual testing, and no association remained significant after false\u2010discovery\u2010rate correction. The effect estimate for Roseburia was not statistically significant in either unadjusted or adjusted analyses. These findings do not support marked differences in overall gut microbiota composition associated with ASD within this\n \n H. pylori<\/jats:italic>\n <\/jats:styled-content>\n \u2010positive cohort; however, the small ASD sample and substantial age imbalance limit the precision and interpretation of the findings.\n <\/jats:p>","DOI":"10.1111\/apm.70271","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T11:51:23Z","timestamp":1791633083000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut Microbiota Composition in\n \n Helicobacter pylori<\/i>\n <\/scp>\n \u2010Positive Adolescents With Autism Spectrum Disorder: An Exploratory Compositional Analysis"],"prefix":"10.1111","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9995-5522","authenticated-orcid":false,"given":"Toshihiko","family":"Kakiuchi","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine Saga University Saga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaroh","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Yamamoto Memorial Hospital Imari Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Imamura Hospital Tosu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0457-0997","authenticated-orcid":false,"given":"Kazutoshi","family":"Hashiguchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Imamura Hospital Tosu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroharu","family":"Kawakubo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Imari Arita Kyouritsu Hospital Arita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Ureshino Medical Center Ureshino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Fujioka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Fujioka Hospital Saga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5263-2868","authenticated-orcid":false,"given":"Masumi","family":"Okuda","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, School of Medicine Hyogo Medical University Nishinomiya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2018"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398\u2010019\u20100389\u20106"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12030792"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12590"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201056631\u20104"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen\u20102020\u2010047941"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442\u20109993.2001.01070.pp.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201028034\u2010z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02224"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517\u20106161.1995.tb02031.x"},{"key":"e_1_2_11_12_1","unstructured":"T.Kakiuchi Y.Tanaka H.Ohno et\u00a0al. \u201c16S rRNA Gene Amplicon Sequencing Data From Japanese Adolescents WithHelicobacter pyloriInfection [Dataset]\u201d DNA Data Bank of Japan (DDBJ) BioProject PRJDB10599; Sequence Read Archive DRP009001 (2021)."}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.70271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apm.70271","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apm.70271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T11:51:25Z","timestamp":1791633085000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apm.70271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":11,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/apm.70271"],"URL":"https:\/\/doi.org\/10.1111\/apm.70271","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70271"}}