{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:43:39Z","timestamp":1792417419865,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["ORF\u20102026\u2010364"],"award-info":[{"award-number":["ORF\u20102026\u2010364"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fungal infections caused by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Candida albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    are challenging to treat with antifungal drugs due to the emergence of antimicrobial\u2010resistant strains, necessitating the development of innovative therapeutic methods. The present study demonstrates that cationic copper sulfide nanoparticles (cCuS) synthesized using [2\u2010((N\u2010(2\u2010hydroxyethyl)palmitamido)methyl)\u20101\u2010methylpyridin\u20101\u2010ium iodide] exhibit increased efficacy against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    when delivered using Jacalin, a lectin from jackfruit that binds to cell surface glycans. The requirement of cCuS to kill was reduced twofold when delivered through Jacalin. Membrane integrity assays showed that JcCuS caused only minimal membrane permeabilization, indicating that membrane disruption is not the primary mechanism of action. Instead, JcCuS significantly increased intracellular reactive oxygen species (ROS), suggesting that oxidative stress plays a central role in its antifungal activity. Elevated ROS levels were markedly reduced by the antioxidant N\u2010acetyl cysteine, confirming ROS\u2010mediated toxicity. Excess ROS also induced apoptosis\u2010associated nuclear condensation and disrupted fungal redox homeostasis by significantly decreasing intracellular reduced glutathione levels. In addition, JcCuS effectively inhibited hyphal growth and filamentation, key virulence factors required for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    pathogenesis. These findings demonstrate that lectin\u2010mediated delivery enhances the efficacy of cCuS nanoparticles by promoting ROS\u2010dependent fungal cell death while preserving membrane integrity, representing a promising strategy for developing targeted antifungal nanotherapeutics.\n                  <\/jats:p>","DOI":"10.1111\/apm.70272","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:05:02Z","timestamp":1792415102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytolectin Potentiates Copper Sulfide Nanoparticles Anticandida Activity Through Enhanced\n                    <scp>ROS<\/scp>\n                    Production"],"prefix":"10.1111","volume":"134","author":[{"given":"Dharshini","family":"Karnan Singaravelu","sequence":"first","affiliation":[{"name":"Department of Chemistry, School of Chemical and Biotechnology SASTRA Deemed University  Thanjavur Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariappan","family":"Mariappan","sequence":"additional","affiliation":[{"name":"Department of Chemistry SRM IST  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1830-7783","authenticated-orcid":false,"given":"Abdullah","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Engineering and Architecture University of Trieste  Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najla A.","family":"Alashaikh","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi\u00a0Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5185-022X","authenticated-orcid":false,"given":"Fuad","family":"Ameen","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi\u00a0Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0931-8192","authenticated-orcid":false,"given":"Anbazhagan","family":"Veerappan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Chemical and Biotechnology SASTRA Deemed University  Thanjavur Tamil Nadu 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