{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:52:43Z","timestamp":1785775963274,"version":"3.56.0"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T00:00:00Z","timestamp":1366588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Summary<\/jats:title><jats:p>Barrett's oesophagus (<jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content>) and gastro\u2010oesophageal reflux disease (<jats:styled-content style=\"fixed-case\">GERD<\/jats:styled-content>) are precursors of oesophageal adenocarcinoma (<jats:styled-content style=\"fixed-case\">OAC<\/jats:styled-content>). There is an oesophageal biofilm, which changes in disease, but its role in aetiopathogenesis remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To define the oesophageal microbiota of patients with <jats:styled-content style=\"fixed-case\">GERD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> BO<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">OAC<\/jats:styled-content> compared with controls and to investigate mucosal responses related to the microbiota.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cultural analysis identified the dominant bacterial species from a subset of each disease group. Based on this, molecular techniques were used to define the cohort. Host responses were analysed in tissues and co\u2010culture experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 111 species belonging to 26 genera were isolated. There was a significant decrease in bacterial counts in the <jats:styled-content style=\"fixed-case\">GERD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content> groups for all genera except <jats:italic>Campylobacter<\/jats:italic>, which colonised <jats:styled-content style=\"fixed-case\">GERD<\/jats:styled-content> and Barrett's patients in increasing numbers. <jats:italic>Campylobacter concisus<\/jats:italic> was the dominant species. This relationship was not seen in the cancer group. Significant increases in <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201018 were seen in <jats:styled-content style=\"fixed-case\">GERD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content> colonised by <jats:italic>Campylobacter<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study defines differences in the oesophageal biofilm in disease states, revealing the emergence of <jats:italic>C. concisus<\/jats:italic> as the dominant new colonist in the refluxed oesophagus. We also associate the presence of these bacteria with increased expression of cytokines related to carcinogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.12317","type":"journal-article","created":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T08:50:38Z","timestamp":1366620638000},"page":"1084-1092","source":"Crossref","is-referenced-by-count":188,"title":["Oesophageal bacterial biofilm changes in gastro\u2010oesophageal reflux disease, Barrett's and oesophageal carcinoma: association or causality?"],"prefix":"10.1111","volume":"37","author":[{"given":"K. L.","family":"Blackett","sequence":"first","affiliation":[{"name":"Gut Group Biomedical Research Institute University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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