{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:27:56Z","timestamp":1790000876172,"version":"4.0.1"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,9]],"date-time":"2016-11-09T00:00:00Z","timestamp":1478649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Technology Support Program","award":["2014BAI08B02"],"award-info":[{"award-number":["2014BAI08B02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neural\u2010immune\u2010endocrine network mechanism has attracted increased attention in diarrhoea\u2010predominant irritable bowel syndrome (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D). Pre\u2010clinical evidence indicates that nerve growth factor (<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>) mediates visceral hypersensitivity and gut barrier dysfunction, via interactions with mast cells and sensory nerve fibres.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To explore the role of nerve growth factor, as well as mast cell\u2013nerve growth factor\u2013nerve interaction in <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D pathophysiology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this cross\u2010sectional study, <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D patients and healthy controls first underwent clinical and psychological assessments. Visceral sensitivity to rectal distension was tested. As gut barrier function markers, serum diamine oxidase and <jats:sc>d<\/jats:sc>\u2010lactate were detected. Rectosigmoid biopsies were taken for the analyses of nerve growth factor expression, mast cell count and activation, and sensory nerve fibres expressing transient receptor potential vanilloid 1 and calcitonin gene\u2010related peptide. Correlations between these parameters were examined in patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirty\u2010eight <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D patients (28 males, 10 females; average age 30.2 years) and 20 healthy controls (12 males, 8 females; average age 26.8 years) participated in the study. The patients presented increased psychological symptoms, visceral hypersensitivity and impaired gut barrier function. <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> gene expression, mast cell count and sensory nerve fibres were significantly increased in the patients (<jats:italic>P<\/jats:italic> &lt; 0.05). In correlation analysis, <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> expression was positively correlated with the disease severity, anxiety and serum diamine oxidase; visceral sensitivity thresholds were negatively associated with <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> expression (Bonferroni corrected <jats:italic>P<\/jats:italic> &lt; 0.0029).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Elevated mucosal <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> may interact with mast cells and sensory nerve fibres, contributing to visceral hypersensitivity and impaired gut barrier function in <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.13848","type":"journal-article","created":{"date-parts":[[2016,11,9]],"date-time":"2016-11-09T21:21:04Z","timestamp":1478726464000},"page":"100-114","source":"Crossref","is-referenced-by-count":67,"title":["Increased expression of nerve growth factor correlates with visceral hypersensitivity and impaired gut barrier function in diarrhoea\u2010predominant irritable bowel syndrome: a preliminary explorative study"],"prefix":"10.1111","volume":"45","author":[{"given":"X. J.","family":"Xu","sequence":"first","affiliation":[{"name":"Graduate School of Peking Union Medical College Chinese Academy of Medical Sciences Beijing China"},{"name":"Gastroenterology Department China\u2010Japan Friendship Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Gastroenterology Department China\u2010Japan Friendship Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Liu","sequence":"additional","affiliation":[{"name":"Jinan Central Hospital Affiliated to Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Pan","sequence":"additional","affiliation":[{"name":"Immunohistochemistry Laboratory of Clinical Medical Research Institute China\u2010Japan Friendship Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. K.","family":"Yao","sequence":"additional","affiliation":[{"name":"Graduate School of Peking Union Medical College Chinese Academy of Medical Sciences Beijing China"},{"name":"Gastroenterology Department China\u2010Japan Friendship Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.11569\/wcjd.v16.i5.563"},{"key":"e_1_2_7_3_1","first-page":"663","article-title":"An epidemiological investigation of irritable bowel syndrome in Shanghai Songjiang communities","volume":"31","author":"Shen F","year":"2011","journal-title":"Chin J Dig"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1207068"},{"key":"e_1_2_7_5_1","first-page":"285","article-title":"Nerve growth factor and irritable bowel syndrome","volume":"19","author":"Wang WL","year":"2010","journal-title":"Chin J Gastroenterol Hepatol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-45106-5_10"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12958"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.05.019"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01339.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2012.01933.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.01.042"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1997.142318000.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.01941.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2044-8341.1959.tb00467.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2044-8260.1967.tb00530.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.02007.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0759-8-10"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1781(89)90047-4"},{"key":"e_1_2_7_19_1","first-page":"245","article-title":"Life event scale","volume":"10","author":"Zhang YL","year":"2005","journal-title":"Handb Behav Med Scales"},{"key":"e_1_2_7_20_1","first-page":"255","article-title":"Trait coping style questionnaire","volume":"10","author":"Jiang QJ","year":"2005","journal-title":"Handb Behav Med Scales"},{"key":"e_1_2_7_21_1","first-page":"1","article-title":"Further explorations for a coping style questionnaire","volume":"8","author":"Jiang QJ","year":"1999","journal-title":"Chin J Behav Med Sci"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2006.03044.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2009.04074.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13074"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2010.11.010"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/S003329171400052X"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i7.616"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.01939.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B1400143"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2009.10.005"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.83"},{"key":"e_1_2_7_33_1","first-page":"117","article-title":"Advances in studies on visceral hypersensitivity of irritable bowel syndrome","volume":"19","author":"Zhou XP","year":"2014","journal-title":"Chin J Gastroenterol"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70099-X"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018885028501"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2008.03759.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2007.03398.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.274.3.G584"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2013.198"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-014-0189-7"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.12829"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-013-0919-6"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.02284.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2012-302093"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.12842"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i11.1732"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v8.i1.168"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.5868"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-011-0145-5"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.3916"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejvs.2005.10.031"},{"key":"e_1_2_7_52_1","first-page":"58","article-title":"Changes of plasma D\u2010lactate, diamine oxidase and endotoxin in patients with liver cirrhosis","volume":"3","author":"Ruan P","year":"2004","journal-title":"Hepatobiliary Pancreat Dis Int"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2003.11.055"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.9.3739"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(96)10058-8"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(01)00314-X"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2003.18.2.204"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.039990"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.02036.x"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2009.04056.x"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B1500181"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2010.04409.x"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2005.02512.x"}],"container-title":["Alimentary Pharmacology &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapt.13848","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapt.13848","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apt.13848","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T16:54:01Z","timestamp":1695488041000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apt.13848"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,9]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1111\/apt.13848"],"URL":"https:\/\/doi.org\/10.1111\/apt.13848","archive":["Portico"],"relation":{},"ISSN":["0269-2813","1365-2036"],"issn-type":[{"value":"0269-2813","type":"print"},{"value":"1365-2036","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,9]]}}}