{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:00:40Z","timestamp":1790719240199,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T00:00:00Z","timestamp":1519084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Severe alcoholic hepatitis patients have high mortality and limited response to corticosteroids. Microvesicles reflect cellular stress and disease conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate whether microvesicles are associated with severity, response to steroid therapy and inflammation in severe alcoholic hepatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Microvesicles originating from different cells were studied pre\u2010therapy in 101 patients; (71 responder to corticosteroid therapy and 30 nonresponders) and 20 healthy controls. Microvesicles and cells were determined in peripheral and hepatic vein samples using flow cytometry and correlated with outcomes. Inflammatory signalling pathways and functional alterations of immune cells after stimulation with microvesicles were also investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Microvesicles mean levels were higher in nonresponders for T cells (CD3<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup>; 10.1 MV\/\u03bcL vs 5.4; <jats:italic>P<\/jats:italic> = 0.06), macrophages (CD68<jats:sup>+<\/jats:sup> CD11b<jats:sup>+<\/jats:sup>; 136.5 vs 121.9 MV\/\u03bcL; <jats:italic>P<\/jats:italic> = 0.01), haematopoietic stem\u2010cells (CD45<jats:sup>+<\/jats:sup> CD34<jats:sup>+<\/jats:sup>; 116.8 vs 13.4 MV\/\u03bcL; <jats:italic>P<\/jats:italic> = 0.0001) and hepatocytes (ASGPR<jats:sup>+<\/jats:sup>; 470 vs 361 MV\/\u03bcL; <jats:italic>P<\/jats:italic> = 0.01); the latter two predicting steroid nonresponse in 94% patients at baseline in peripheral plasma. Microvesicle levels correlated with histological and liver disease severity indices. Whereas, in non\u2010responders hepatic vein CD34+ cells were lower (<jats:italic>P<\/jats:italic> = 0.02), the CD34+ microvesicles there from were higher (<jats:italic>P<\/jats:italic> = 0.04), thus suggesting impaired regeneration. Also, microvesicles of 0.2\u20100.4 \u03bcm size were higher in nonresponders (<jats:italic>P<\/jats:italic> &lt; 0.03) at baseline. Microvesicles from patients trigger more (<jats:italic>P<\/jats:italic> = 0.04) <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> generation, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 production (<jats:italic>P<\/jats:italic> = 0.04) and up\u2010regulate pro\u2010inflammatory cytokine related genes in neutrophils in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Pre\u2010therapy peripheral plasma levels of CD34<jats:sup>+<\/jats:sup> and ASGPR<jats:sup>+<\/jats:sup> microvesicles are reliable non\u2010invasive markers of steroid nonresponse and mortality in patients with severe alcoholic hepatitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.14564","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T18:58:24Z","timestamp":1519239504000},"page":"1151-1161","source":"Crossref","is-referenced-by-count":37,"title":["Microvesicles in hepatic and peripheral vein can predict nonresponse to corticosteroid therapy in severe alcoholic hepatitis"],"prefix":"10.1111","volume":"47","author":[{"given":"S.","family":"Sukriti","sequence":"first","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. S.","family":"Maras","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Bihari","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6333-0195","authenticated-orcid":false,"given":"A. K.","family":"Vyas","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Shasthry","sequence":"additional","affiliation":[{"name":"Department of Hepatology Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Department of Hepatology Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Premkumar","sequence":"additional","affiliation":[{"name":"Department of Hepatology Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Special center for molecular medicine JNU Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6109-0033","authenticated-orcid":false,"given":"N.","family":"Trehanpati","sequence":"additional","affiliation":[{"name":"Department of Molecular and cellular medicine Institute of Liver and Biliary Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. E.","family":"Rautou","sequence":"additional","affiliation":[{"name":"Department of Hepatology Hopital Beaujon INSERM U970 University of Paris Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Moreau","sequence":"additional","affiliation":[{"name":"Center for Research on Inflammation Xavier Bichat School of Medicine INSERM U1149 Paris\u2010Diderot University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0544-5610","authenticated-orcid":false,"given":"S. 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