{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:24:21Z","timestamp":1740137061939,"version":"3.37.3"},"reference-count":39,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2018,11,8]],"date-time":"2018-11-08T00:00:00Z","timestamp":1541635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Zymogen granule glycoprotein 2 (GP2) is a major autoantigen of Crohn's disease\u2010specific pancreatic autoantibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To test a link between loss of tolerance to isoforms of GP2 and pouch disorders in a cross\u2010sectional study in ulcerative colitis patients with ileal pouch\u2010anal anastomosis (IPAA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum samples of 117 consecutive ulcerative colitis patients after IPAA were tested for presence of Anti\u2010GP2 isoforms 1 (GP2<jats:sub>1<\/jats:sub>) &amp; 4 (GP2<jats:sub>4<\/jats:sub>) IgG and IgA as well as anti\u2010<jats:italic>Saccaromyces cervisiae<\/jats:italic> (ASCA) IgG and IgA antibodies in a blinded fashion via enzyme\u2010linked immunosorbent assay. Pouch disorders were diagnosed based on clinical, endoscopic, histological and radiographic criteria. Crohn's disease of the pouch was defined as involvement of the small bowel mucosa proximal to the ileal pouch with Crohn's disease, development of perianal complications or pouch fistula more than 3\u00a0months after ileostomy closure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Positivity and level of Anti\u2010GP2<jats:sub>1<\/jats:sub> IgG (AUC 0.77; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001 &amp; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02, respectively), Anti\u2010GP2<jats:sub>4<\/jats:sub> IgG (AUC 0.74; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001 &amp; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.025, respectively) and Anti\u2010GP2<jats:sub>4<\/jats:sub> IgA (AUC 0.77; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001 to <jats:italic>P<\/jats:italic>\u00a0=\u00a00.018, respectively) were specifically associated with Crohn's disease of the pouch. Anti\u2010GP2 was not associated with endoscopic or histological pouch disease activity index. Neither positivity nor levels of ASCA IgG (AUC 0.63; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.12 &amp; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.35, respectively) or ASCA IgA (AUC 0.67; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.38 &amp; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.53) were associated with pouch phenotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The novel anti\u2010GP2<jats:sub>1<\/jats:sub> and GP2<jats:sub>4<\/jats:sub> antibodies are associated with Crohn's disease of the pouch in ulcerative colitis patients after IPAA. Serological anti\u2010GP2 antibodies could aid in diagnosis of Crohn's disease of the pouch.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.15034","type":"journal-article","created":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T02:01:05Z","timestamp":1541728865000},"page":"1251-1259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Loss of tolerance to glycoprotein 2 isoforms 1 and 4 is associated with Crohn\u2019s disease of the pouch"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4400-3540","authenticated-orcid":false,"given":"Donelle","family":"Cummings","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Medicine Institute, Cleveland Clinic Foundation Cleveland Ohio"},{"name":"Division of Gastroenterology and Hepatology, Department of Medicine SUNY Downstate Medical Center Brooklyn New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Cruise","sequence":"additional","affiliation":[{"name":"Department of Anatomic Pathology, Pathology and Laboratory Medicine Institute Cleveland Clinic Foundation Cleveland Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rocio","family":"Lopez","sequence":"additional","affiliation":[{"name":"Quantitative Health Sciences Lerner Research Institute, Cleveland Clinic Foundation Cleveland Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Roggenbuck","sequence":"additional","affiliation":[{"name":"Faculty Environment and Natural Sciences Brandenburg University of Technology Cottbus\u2010Senftenberg Senftenberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vipul","family":"Jairath","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Western Ontario London ON Canada"},{"name":"Department of Epidemiology and Biostatistics University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5148-6130","authenticated-orcid":false,"given":"Yinghong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology &amp; 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