{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:02:23Z","timestamp":1775977343725,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The appropriate location for biopsy procurement relative to an ulcer in active Crohn's disease is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To explore the relationship between biopsy location, histological disease activity, proinflammatory gene expression and the presence of inflammatory cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifty\u2010one patients with Crohn's disease and ulcers &gt;0.5\u00a0cm diameter in the colon and\/or ileum were prospectively enrolled at three centres. Biopsies were obtained from 0\u00a0mm, 7 to 8\u00a0mm and 21 to 24\u00a0mm from the edge of the largest ulcer. Histological activity was blindly assessed with the Global Histological Disease Activity Score, the Robarts Histopathology and Nancy Histological indices. Messenger ribonucleic acid (mRNA) levels for interleukins\u20106, \u20108 and \u201023 (p19 and p40 subunits), CD31 and S100A9 were measured using quantitative polymerase chain reaction. The number of CD3+, CD68+ and myeloperoxidase\u2010positive cells was quantified by immunohistochemistry. Data were analysed using mixed models with location and segment as fixed effects and patients as random effect to account for correlation among segments within a patient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histological disease activity scores (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001), proinflammatory gene expression levels (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.005) and numbers of myeloperoxidase\u2010positive cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) were highest in biopsies from the ulcer edge in the colon and ileum, with decreasing gradients observed with distance from the edge (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). No differences between colonic and ileal samples were detected for the parameters measured at any location.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Biopsies from the ulcer edge in patients with Crohn's disease yielded the greatest histological disease activity and mRNA levels and had similar readouts in the colon and ileum. Research is needed to confirm this conclusion for other measures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.15250","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T07:22:31Z","timestamp":1555312951000},"page":"1401-1409","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Evaluation of optimal biopsy location for assessment of histological activity, transcriptomic and immunohistochemical analyses in patients with active Crohn\u2019s disease"],"prefix":"10.1111","volume":"49","author":[{"given":"Gregor","family":"Novak","sequence":"first","affiliation":[{"name":"Robarts Clinical Trials Inc London ON Canada"},{"name":"Department of Gastroenterology Ljubljana University Medical Centre, University of Ljubljana Ljubljana 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